Rabbit TIMP3 Polyclonal Antibody | anti-TIMP3 antibody

Anti-TIMP3 Antibody

Cat. Num. AAA416708

• Gene Names: TIMP3; SFD; K222; K222TA2; HSMRK222
• Reactivity: Human. Not yet tested in other species
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Affinity purified
• Synonyms: TIMP3; Polyclonal Antibody; Anti-TIMP3 Antibody; anti-TIMP3 antibody

• Host: Rabbit
• Reactivity: Human. Not yet tested in other species
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 211

• Applicable Applications for anti-TIMP3 antibody: ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Peptide ELISA titer: 1:5,000-1:20,000; Immunohistochemistry: 1:100-500; Optimal dilutions/concentrations should be determined by the end user
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Rabbit polyclonal TIMP3 antibody was raised against a peptide from the intermediate residues of human TIMP3 protein.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-TIMP3 antibody

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. [provided by RefSeq, Jul 2008]

References

(1) Wisniewska,M., Goettig,P., Maskos,K., et al.Structural determinants of the ADAM inhibition by TIMP-3: crystal structure of the TACE-N-TIMP-3 complex. J. Mol. Biol. 381 (5), 1307-1319 (2008)

NCBI and Uniprot Product Information

• NCBI GI #: 47678717
• NCBI GeneID: 7078
• UniProt Accession #: P35625; Q5THV4; Q9UC74; Q9UGS2; B2RBY9
• Molecular Weight: 24,145 Da
• NCBI Official Full Name: TIMP3
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 3
• NCBI Official Symbol: TIMP3
• NCBI Official Synonym Symbols: SFD; K222; K222TA2; HSMRK222
• NCBI Protein Information: metalloproteinase inhibitor 3; MIG-5 protein; TIMP-3; protein MIG-5; tissue inhibitor of metalloproteinases 3
• UniProt Protein Name: Metalloproteinase inhibitor 3
• Protein Family: Metalloproteinase inhibitor
• UniProt Gene Name: TIMP3
• UniProt Entry Name: TIMP3_HUMAN

NCBI Description

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. [provided by RefSeq, Jul 2008]

Uniprot Description

TIMP3: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15. Interacts with EFEMP1. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; basement membrane; nucleus

Molecular Function: metalloendopeptidase inhibitor activity; protein binding; protease binding; metal ion binding

Biological Process: negative regulation of metalloenzyme activity; visual perception; response to cytokine stimulus; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis

Disease: Fundus Dystrophy, Pseudoinflammatory, Of Sorsby