Rabbit anti-Human TIMP1 Polyclonal Antibody | anti-TIMP1 antibody

TIMP1, CT (TIMP1, CLGI, TIMP, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, Tissue inhibitor of metalloproteinases 1)

Cat. Num. AAA6006868

• Gene Names: TIMP1; EPA; EPO; HCI; CLGI; TIMP
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: TIMP1; Polyclonal Antibody; CT (TIMP1; CLGI; TIMP; Metalloproteinase inhibitor 1; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; Tissue inhibitor of metalloproteinases 1); Anti -TIMP1; anti-TIMP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-TIMP1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:50-100; Immunohistochemistry: 1:50-100; Immunofluorescence: 1:10-50; Flow Cytometry: 1:10-50
• Immunogen: TIMP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164-195 amino acids from the C-terminal region of human TIMP1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TIMP1 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the TIMP1 antibody detected the TIMP1 protein (arrow).)

Immunohistochemistry (IHC)

(TIMP1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TIMP1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of TIMP1 Antibody (C-term) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of TIMP1 Antibody (C-term) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)

Flow Cytometry (FC/FACS)

(TIMP1 Antibody (C-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TIMP1 antibody

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction.

Product Categories/Family for anti-TIMP1 antibody

Antibodies; Abs to Matrix Metalloproteinases

NCBI and Uniprot Product Information

• NCBI GI #: 49456917
• NCBI GeneID: 7076
• UniProt Accession #: Q6FGX5; P01033
• Molecular Weight: 23,171 Da
• NCBI Official Full Name: TIMP1
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 1
• NCBI Official Symbol: TIMP1
• NCBI Official Synonym Symbols: EPA; EPO; HCI; CLGI; TIMP
• NCBI Protein Information: metalloproteinase inhibitor 1; TIMP-1; collagenase inhibitor; erythroid potentiating activity; erythroid-potentiating activity; fibroblast collagenase inhibitor; tissue inhibitor of metalloproteinases 1
• UniProt Protein Name: TIMP metallopeptidase inhibitor 1, isoform CRA_a
• Protein Family: Metalloproteinase inhibitor
• UniProt Gene Name: TIMP1
• UniProt Entry Name: Q6FGX5_HUMAN

NCBI Description

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008]

Uniprot Description

TIMP1: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xp11.3-p11.23

Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region; basement membrane

Molecular Function: metalloendopeptidase inhibitor activity; protein binding; protease binding; growth factor activity; metal ion binding; cytokine activity

Biological Process: response to peptide hormone stimulus; extracellular matrix disassembly; platelet activation; negative regulation of metalloenzyme activity; extracellular matrix organization and biogenesis; platelet degranulation; response to cytokine stimulus; positive regulation of cell proliferation; response to hormone stimulus; blood coagulation; negative regulation of membrane protein ectodomain proteolysis; aging; negative regulation of apoptosis