Western Blot (WB)
(Western blot analysis of ALKBH2 using HT29 whole cell lysates)
Rabbit anti-Human, Mouse ALKBH2 Polyclonal Antibody | anti-ALKBH2 antibody
ALKBH2 Antibody
Gene Names
ALKBH2; ABH2
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ALKBH2; Polyclonal Antibody; ALKBH2 Antibody; 2OG Fe(II) oxy DC1; 9530023G02; ABH 2; ABH2; alkB alkylation repair homolog 2; ALKB2_HUMAN; ALKBH2 alkB; Alkylated DNA repair protein alkB homolog; Alkylated DNA repair protein alkB homolog 2; Alkylation repair homolog 2; Alpha ketoglutarate dependent dioxygenase alkB homolog 2; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2; AU016977; DNA oxidative demethylase ALKBH2; EC 1.14.11.; FLJ99103; hABH2; mABH2; MGC90512; Oxy DC1; anti-ALKBH2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
ALKBH2 antibody detects endogenous levels of total ALKBH2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
261
Applicable Applications for anti-ALKBH2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human ALKBH2
Subcellular Location
Nucleus. Detected in replication foci during S-phase.
Tissue Specificity
Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (91%), Zebrafish (91%), Bovine (91%), Horse (91%), Sheep (91%), Rabbit (91%), Dog (100%), Chicken (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of ALKBH2 using HT29 whole cell lysates)
Western Blot (WB)
(Western blot analysis of ALKBH2 using HT29 whole cell lysates)
Related Product Information for anti-ALKBH2 antibody
Function: Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.
Similarity: Belongs to the alkB family.
Similarity: Belongs to the alkB family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 29 kDa
Predicted: 30 kDa
Predicted: 30 kDa
NCBI Official Full Name
DNA oxidative demethylase ALKBH2 isoform 1
NCBI Official Synonym Full Names
alkB homolog 2, alpha-ketoglutarate dependent dioxygenase
NCBI Official Symbol
ALKBH2
NCBI Official Synonym Symbols
ABH2
NCBI Protein Information
DNA oxidative demethylase ALKBH2
UniProt Protein Name
DNA oxidative demethylase ALKBH2
Protein Family
UniProt Gene Name
ALKBH2
UniProt Synonym Gene Names
ABH2
NCBI Description
The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 and ALKBH3 (MIM 610603) are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM, Mar 2008]
Uniprot Description
Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.
Research Articles on ALKBH2
Similar Products
Product Notes
The ALKBH2 alkbh2 (Catalog #AAA9609390) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ALKBH2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ALKBH2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the ALKBH2 alkbh2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALKBH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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