Western Blot (WB)
((0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat TIMP1 Polyclonal Antibody | anti-TIMP1 antibody
Goat anti-TIMP1 (aa129-143) Antibody
Western Blot: Approx 24kDa band observed in Human Kidney and Liver lysates (calculated MW of 23.2kDa according to NP_003245.1). Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
((0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008]
Uniprot Description
TIMP1: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. Belongs to the protease inhibitor I35 (TIMP) family.
Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: Xp11.3-p11.23
Cellular Component: basement membrane; extracellular region; extracellular space; proteinaceous extracellular matrix
Molecular Function: cytokine activity; growth factor activity; metal ion binding; metalloendopeptidase inhibitor activity; protease binding; protein binding
Biological Process: aging; cartilage development; cell activation; extracellular matrix disassembly; negative regulation of apoptosis; negative regulation of membrane protein ectodomain proteolysis; platelet degranulation; positive regulation of cell proliferation; response to cytokine stimulus; response to hormone stimulus; response to peptide hormone stimulus; wound healing
Research Articles on TIMP1
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Product Notes
The TIMP1 timp1 (Catalog #AAA422816) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-TIMP1 (aa129-143) Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Dog, and may cross-react with other species as described in the data sheet. AAA Biotech's TIMP1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 24kDa band observed in Human Kidney and Liver lysates (calculated MW of 23.2kDa according to NP_003245.1). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the TIMP1 timp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NSLSLAQRRG FTKTY. It is sometimes possible for the material contained within the vial of "TIMP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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