IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with COT Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit COT (MAP3K8/MEKK8) Polyclonal Antibody | anti-MAP3K8 antibody
COT (MAP3K8/MEKK8) Antibody (C-term)
Gene Names
MAP3K8; COT; EST; ESTF; TPL2; AURA2; MEKK8; Tpl-2; c-COT
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
COT (MAP3K8/MEKK8), Antibody; COT (MAP3K8/MEKK8) Antibody (C-term); Mitogen-activated protein kinase kinase kinase 8; Cancer Osaka thyroid oncogene; Proto-oncogene c-Cot; Serine/threonine-protein kinase cot; Tumor progression locus 2; TPL-2; MAP3K8; COT; ESTF; anti-MAP3K8 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This COT (MAP3K8/MEKK8) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 368-397 amino acids from the C-terminal region of human COT (MAP3K8/MEKK8).
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
368-397
Sequence Length
467
Applicable Applications for anti-MAP3K8 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with COT Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with COT Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(The anti-COT Pab is used in Western blot to detect COT in HeLa cell lysate.)
WB (Western Blot)
(The anti-COT Pab is used in Western blot to detect COT in HeLa cell lysate.)
Related Product Information for anti-MAP3K8 antibody
COT, a member of the MAPKKK subfamily of Ser/Thr protein kinases, is able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. It plays a role in the cell cycle. The longer form of cot has some transforming activity, although it is much weaker than the activated cot oncoprotein. This cytoplasmic protein is expressed in several normal tissues and human tumor-derived cell lines. The 58 kDa form is activated specifically during the S and G2/M phases of the cell cycle. The longer form undergoes phosphorylation on Ser residues mainly, and the shorter form on both Ser and Thr residues.
Product Categories/Family for anti-MAP3K8 antibody
References
Sanchez-Gongora, E., et al., J. Biol. Chem. 275(40):31379-31386 (2000).
Aoki, M., et al., J. Biol. Chem. 268(30):22723-22732 (1993).
Chan, A.M., et al., Oncogene 8(5):1329-1333 (1993).
Miyoshi, J., et al., Mol. Cell. Biol. 11(8):4088-4096 (1991).
Aoki, M., et al., Oncogene 6(9):1515-1519 (1991).
Aoki, M., et al., J. Biol. Chem. 268(30):22723-22732 (1993).
Chan, A.M., et al., Oncogene 8(5):1329-1333 (1993).
Miyoshi, J., et al., Mol. Cell. Biol. 11(8):4088-4096 (1991).
Aoki, M., et al., Oncogene 6(9):1515-1519 (1991).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52925
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase 8
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 8
NCBI Official Symbol
MAP3K8
NCBI Official Synonym Symbols
COT; EST; ESTF; TPL2; AURA2; MEKK8; Tpl-2; c-COT
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 8
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 8
UniProt Gene Name
MAP3K8
UniProt Synonym Gene Names
COT; ESTF; TPL-2
UniProt Entry Name
M3K8_HUMAN
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Product Notes
The MAP3K8 map3k8 (Catalog #AAA288655) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 368-397. The COT (MAP3K8/MEKK8) Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's COT (MAP3K8/MEKK8) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MAP3K8 map3k8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COT (MAP3K8/MEKK8), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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