Rat Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) RTU ELISA Kit | PPM1A rtu elisa kit

Rat Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) ELISA Kit

Cat. Num. AAA4501989

• Gene Names: Ppm1a; Pp2c1
• Reactivity: Rat
• Synonyms: Protein Phosphatase; Mg2+/Mn2+ Dependent 1A (PPM1A); Rat Protein Phosphatase; Mg2+/Mn2+ Dependent 1A (PPM1A) ELISA Kit; PP2C-ALPHA; PP2CA; Phosphatase 2C Alpha; Protein Phosphatase 1A(Formerly 2C)Magnesium-Dependent; Alpha; PPM1A rtu elisa kit

• Reactivity: Rat
• Sequence Length: 382

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.138ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PPM1A rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PPM1A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PPM1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PPM1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PPM1A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 8394012
• NCBI GeneID: 24666
• NCBI Accession #: NP_058734.1
• NCBI GenBank Nucleotide #: NM_017038.1
• UniProt Accession #: P20650
• Molecular Weight: 42,417 Da
• NCBI Official Full Name: protein phosphatase 1A
• NCBI Official Synonym Full Names: protein phosphatase, Mg2+/Mn2+ dependent, 1A
• NCBI Official Symbol: Ppm1a
• NCBI Official Synonym Symbols: Pp2c1
• NCBI Protein Information: protein phosphatase 1A
• UniProt Protein Name: Protein phosphatase 1A
• Protein Family: Protein phosphatase
• UniProt Gene Name: Ppm1a
• UniProt Synonym Gene Names: Pp2c1; Pppm1a; PP2C-alpha

Uniprot Description

Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling (). Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB ().