Rabbit anti-Human, Mouse TIGIT Polyclonal Antibody | anti-TIGIT antibody

TIGIT Antibody

Cat. Num. AAA9217285

• Gene Names: TIGIT; VSIG9; VSTM3; WUCAM
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Synonyms: TIGIT; Polyclonal Antibody; TIGIT Antibody; T-cell immunoreceptor with Ig and ITIM domains; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain-containing protein 3; VSIG9; VSTM3; anti-TIGIT antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This TIGIT antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 12-142 amino acids from human TIGIT.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 244

• Applicable Applications for anti-TIGIT antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB:~~1:2000
• Function: Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.Cellular Location
• Tissue Location: Expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level).
• Cellular Location: Cell membrane; Single-pass type I membrane protein.
• Preparation and Storage: Maintain refrigerated at 2-8°C. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-TIGIT Antibody at 1:2000 dilutionLane 1: EL4 whole cell lysateLane 2: human fetal thymus lysateLane 3: human spleen lysateLane 4: HUT78 whole cell lysateLane 5: Jurkat whole cell lysateLane 6: MOLT-4 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-TIGIT antibody

Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

Product Categories/Family for anti-TIGIT antibody

Primary Antibodies; Immunology

References

Ota T.,et al.Nat. Genet. 36:40-45 (2004). Bechtel S.,et al.BMC Genomics 8:399-399 (2007). Yu X.,et al.Nat. Immunol. 10:48-57 (2009). Stengel K.F.,et al.Proc. Natl. Acad. Sci. U.S.A. 109:5399-5404 (2012).

NCBI and Uniprot Product Information

• NCBI GI #: 256600228
• NCBI GeneID: 201633
• NCBI Accession #: NP_776160.2
• NCBI GenBank Nucleotide #: NM_173799.3
• UniProt Accession #: Q495A1; Q495A3; Q5JPD8; Q6MZS2; Q8N877
• Molecular Weight: 26319
• NCBI Official Full Name: T-cell immunoreceptor with Ig and ITIM domains
• NCBI Official Synonym Full Names: T-cell immunoreceptor with Ig and ITIM domains
• NCBI Official Symbol: TIGIT
• NCBI Official Synonym Symbols: VSIG9; VSTM3; WUCAM
• NCBI Protein Information: T-cell immunoreceptor with Ig and ITIM domains
• UniProt Protein Name: T-cell immunoreceptor with Ig and ITIM domains
• Protein Family: T-cell immunoreceptor with Ig and ITIM domains
• UniProt Gene Name: TIGIT
• UniProt Synonym Gene Names: VSIG9; VSTM3
• UniProt Entry Name: TIGIT_HUMAN

NCBI Description

This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.[provided by RefSeq, Sep 2009]

Uniprot Description

TIGIT: Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q13.31

Cellular Component: cell surface; cell-cell adherens junction; integral to plasma membrane

Molecular Function: cell adhesion molecule binding; protein binding; protein homodimerization activity; receptor activity; receptor binding

Biological Process: cell recognition; heterophilic cell adhesion; homophilic cell adhesion; negative regulation of interleukin-12 production; negative regulation of T cell activation; positive regulation of interleukin-10 production