Rabbit anti-Human TICAM-1 Polyclonal Antibody | anti-TICAM1 antibody

TICAM-1 (Toll-interleukin 1 Receptor Domain (TIR)-containing Adaptor Molecule-1, TRIF, Toll/IL-1 Receptor Domain-containing Adapter-inducing IFN-beta)

Cat. Num. AAA614357

• Gene Names: TICAM1; TRIF; PRVTIRB; TICAM-1; MGC35334
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Highly Purified; Purified by Ion Exchange chromatography.
• Synonyms: TICAM-1; Polyclonal Antibody; TICAM-1 (Toll-interleukin 1 Receptor Domain (TIR)-containing Adaptor Molecule-1; TRIF; Toll/IL-1 Receptor Domain-containing Adapter-inducing IFN-beta); Anti -TICAM-1 (Toll-interleukin 1 Receptor Domain (TIR)-containing Adaptor Molecule-1; anti-TICAM1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TRIF (TICAM-1).
• Purity/Purification: Highly Purified; Purified by Ion Exchange chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

• Applicable Applications for anti-TICAM1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.
• Immunogen: Synthetic peptide corresponding to 14aa near the C-terminus of human TRIF. GenBank accession #NP_891549.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of TRIF in human lung cell lysates with MBS614357 at (A) 2 and (B) 4 ug/ml.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of MBS614357.)

Related Product Information for anti-TICAM1 antibody

TRIF is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs) (1-3). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses (4). TRIF, along with other molecules such as TIRP, TIRAP, and MyD88, serves as an adaptor protein to several of the TLR molecules. Following activation of TLR3 and TLR4, TRIF engages the kinase TBK1 and allows its subsequent activation of the interferon regulatory factor (IRF)-3 (5). TRIF is also involved in the activation of TNF receptor associated factor (TRAF)-6, and ultimately the activation of NF-kB (6).

Product Categories/Family for anti-TICAM1 antibody

Antibodies; Abs to Adapter Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 41281981
• NCBI GeneID: 148022
• NCBI Accession #: NP_891549.1
• NCBI GenBank Nucleotide #: NM_182919.2
• UniProt Accession #: Q8IUC6; O75532; Q86XP8; Q96GA0; B3Y691
• Molecular Weight: 76,422 Da
• NCBI Official Full Name: TIR domain-containing adapter molecule 1
• NCBI Official Synonym Full Names: toll-like receptor adaptor molecule 1
• NCBI Official Symbol: TICAM1
• NCBI Official Synonym Symbols: TRIF; PRVTIRB; TICAM-1; MGC35334
• NCBI Protein Information: TIR domain-containing adapter molecule 1; TIR domain-containing adaptor molecule 1; putative NF-kappa-B-activating protein 502H; TIR domain containing adaptor inducing interferon-beta; TIR domain-containing adapter protein inducing IFN-beta; proline-rich, vinculin and TIR domain-containing protein B; toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta
• UniProt Protein Name: TIR domain-containing adapter molecule 1
• Protein Family: TIR domain-containing adapter molecule
• UniProt Gene Name: TICAM1
• UniProt Synonym Gene Names: PRVTIRB; TRIF
• UniProt Entry Name: TCAM1_HUMAN

NCBI Description

TRIF, or TICAM1, is a Toll/IL1R (MIM 147810) (TIR) domain-containing adaptor molecule, like MYD88 (MIM 602170) and TIRAP (MIM 606252), that induces interferon-beta (IFNB; MIM 147640), specifically interacts with TLR3 (MIM 603029), and activates nuclear factor kappa-B (NFKB; see MIM 164011).[supplied by OMIM]

Uniprot Description

TICAM1: an adaptor protein involved in innate immunity against invading pathogens. Associates with TLR3 and TLR4 (through TICAM2) to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TICAM1 recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively. Interacts with the TIR domain of TLR3. Interacts with AZI2, TBK1, IRF3 and IRF7. Interacts with TICAM2 in TLR4 recruitment. Interaction with PIAS4 inhibits the TICAM1-induced NF-kappa-B, IRF and IFNB1 activation. Interacts with IKBKB and IKBKE. Interaction with SARM1 blocks TICAM1-dependent transcription factor activation. Interacts with TRAF3. Interacts with TRAFD1.

Protein type: Adaptor/scaffold; Apoptosis

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: endosome membrane; cytosol

Molecular Function: protein binding; signal transducer activity; protein kinase binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of nitric oxide biosynthetic process; positive regulation of protein binding; MyD88-independent toll-like receptor signaling pathway; regulation of protein homodimerization activity; positive regulation of interleukin-6 production; toll-like receptor 3 signaling pathway; positive regulation of tumor necrosis factor production; positive regulation of natural killer cell activation; activation of NF-kappaB transcription factor; positive regulation of chemokine biosynthetic process; response to exogenous dsRNA; positive regulation of protein ubiquitination; macrophage activation during immune response; positive regulation of interferon-beta biosynthetic process; lipopolysaccharide-mediated signaling pathway; toll-like receptor signaling pathway; positive regulation of B cell proliferation; innate immune response; inflammatory response; toll-like receptor 4 signaling pathway; defense response to virus

Disease: Herpes Simplex Encephalitis, Susceptibility To, 4