Rabbit anti-Human, Mouse TIA1 Polyclonal Antibody | anti-TIA1 antibody

TIA1 Polyclonal Antibody

Cat. Num. AAA3013570

• Reactivity: Human, Mouse
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
• Purity: ProA affinity purified
• Synonyms: TIA1; Polyclonal Antibody; TIA1 Polyclonal Antibody; TIA-1; Cytotoxic granule associated RNA binding protein 1; Cytotoxic granule associated RNA binding protein; mTIA-1; Nucleolysin TIA 1 isoform p40; Nucleolysin TIA-1 isoform p40; Nucleolysin TIA1 isoform p40; p40 TIA 1; p40-TIA-1 (containing p15-TIA-1); p40-TIA-1; RNA binding protein TIA 1; RNA binding protein TIA1; RNA-binding protein TIA-1; T-cell-restricted intracellular antigen-1; TIA 1; TIA 1 cytotoxic granule associated RNA binding protein; Tia; TIA1 cytotoxic granule associated RNA binding protein; TIA1 cytotoxic granule associated RNA binding protein like 1; TIA1 protein; TIAL1; TIAR; WDM; anti-TIA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TIA1 polyclonal antibody detects endogenous levels of TIA1 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 386

• Applicable Applications for anti-TIA1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1,000; ICC: 1:50-1:200; IHC: 1:50-1:200; IP: 1:10-1:50
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of TIA1 on Jurkat cells lysates using anti-TIA1 antibody at 1/500 dilution.)

Immunocytochemistry (ICC)

(ICC staining TIA1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-TIA1 antibody

FAS, also referred to as CD95 or APO-1, is a type I transmembrane protein that plays a central role mediating viral immunity. TIA-1 and TIAR are two closely related proteins that possess three RRMs (RNA recognition motifs), designated RRM 1, 2 and 3. Although both TIA-1 and TIAR are thought to function as mediators of apoptotic cell death, their specific roles in such pathways are unknown. Unlike TIA-1, which is found in the granules of cytotoxic lymphocytes, TIAR expression is limited to the nucleus and found in a much broader range of cells including, but not limited to, cells of hematopoietic origin. TIAR is translocated to the cytoplasm shortly after FAS ligation and this event immediately proceeds the onset of DNA fragmentation. A novel serine/threonine kinase that is activated as a result of FAS ligation, designated FAST (FAS-activated serine/threonine), shows kinase specificity towards both TIA-1 and TIAR. In unstimulated Jurkat cells, FAST resides in the cytoplasm as a highly phosphorylated protein and is quickly dephosphorylated and activated in response to stimulated FAS.

NCBI and Uniprot Product Information

• NCBI GI #: 206729905
• UniProt Accession #: P31483; P52912
• Molecular Weight: 43kDa
• NCBI Official Full Name: Nucleolysin TIA-1 isoform p40
• UniProt Protein Name: Nucleolysin TIA-1 isoform p40
• Protein Family: Nucleolysin
• UniProt Gene Name: TIA1
• UniProt Synonym Gene Names: TIA-1
• UniProt Entry Name: TIA1_HUMAN

Uniprot Description

TIA1: Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation; RNA-binding

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: nucleoplasm; stress granule; cytoplasm

Molecular Function: protein binding; nucleotide binding; AU-rich element binding; poly(A) binding

Biological Process: regulation of nuclear mRNA splicing, via spliceosome; apoptosis; negative regulation of translation; negative regulation of cytokine biosynthetic process

Disease: Welander Distal Myopathy