Rabbit anti-Human GRP Polyclonal Antibody | anti-GRP antibody

GRP Antibody (Center)

Cat. Num. AAA9216231

• Gene Names: GRP; BN; GRP-10; proGRP; preproGRP
• Reactivity: Human
• Applications: Western Blot
• Synonyms: GRP; Polyclonal Antibody; GRP Antibody (Center); Purified Rabbit Polyclonal Antibody (Pab); Gastrin-releasing peptide; Neuromedin-C; GRP-10; anti-GRP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This GRP antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 67-97 amino acids from the Central region of human GRP.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 141

• Applicable Applications for anti-GRP antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:2000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-GRP Antibody (Center) at 1:2000 dilutionLane 1: HL-60 whole cell lysateLane 2: PC-3 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 16 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-GRP antibody

GRP stimulates gastrin release as well as other gastrointestinal hormones. Operates as a negative feedback regulating fear and established a causal relationship between GRP- receptor gene expression, long-term potentiation, and amygdala- dependent memory for fear (By similarity).

Product Categories/Family for anti-GRP antibody

Neuroscience; Signal Transduction

References

Spindel E.R.,et al.Proc. Natl. Acad. Sci. U.S.A. 83:19-23(1986). Lebacq-Verheyden A.-M.,et al.Mol. Cell. Biol. 8:3129-3135(1988). Sausville E.A.,et al.J. Biol. Chem. 261:2451-2457(1986). Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases. Nusbaum C.,et al.Nature 437:551-555(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 60498997
• NCBI GeneID: 2922
• NCBI Accession #: NP_001012530.1
• NCBI GenBank Nucleotide #: NM_001012512.1
• UniProt Accession #: P07492; P07491; P81553; Q14454; Q53YA0; Q9BSY7
• Molecular Weight: 16213
• NCBI Official Full Name: gastrin-releasing peptide isoform 2 preproprotein
• NCBI Official Synonym Full Names: gastrin-releasing peptide
• NCBI Official Symbol: GRP
• NCBI Official Synonym Symbols: BN; GRP-10; proGRP; preproGRP
• NCBI Protein Information: gastrin-releasing peptide
• UniProt Protein Name: Gastrin-releasing peptide
• Protein Family: GrpE protein
• UniProt Gene Name: GRP
• UniProt Synonym Gene Names: GRP
• UniProt Entry Name: GRP_HUMAN

NCBI Description

This gene encodes a member of the bombesin-like family of gastrin-releasing peptides. Its preproprotein, following cleavage of a signal peptide, is further processed to produce either the 27 aa gastrin-releasing peptide or the 10 aa neuromedin C. These smaller peptides regulate numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation. These peptides are also likely to play a role in human cancers of the lung, colon, stomach, pancreas, breast, and prostate. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

GRP: GRP stimulates gastrin release as well as other gastrointestinal hormones. Operates as a negative feedback regulating fear and established a causal relationship between GRP- receptor gene expression, long-term potentiation, and amygdala- dependent memory for fear. Belongs to the bombesin/neuromedin-B/ranatensin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 18q21.1-q21.32

Cellular Component: extracellular space; extracellular region

Molecular Function: neuropeptide hormone activity; receptor binding

Biological Process: neuropeptide signaling pathway; signal transduction