Rabbit THOC4 Polyclonal Antibody | anti-ALYREF antibody

THOC4 Antibody

Cat. Num. AAA9211031

• Gene Names: ALYREF; ALY; BEF; REF; THOC4; ALY/REF
• Reactivity: Human, mouse, rat
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: THOC4; Polyclonal Antibody; THOC4 Antibody; THO complex subunit 4; Tho4; Ally of AML-1 and LEF-1; Aly/REF export factor; Transcriptional coactivator Aly/REF; bZIP-enhancing factor BEF; ALYREF; ALY; BEF; anti-ALYREF antibody

• Host: Rabbit
• Reactivity: Human, mouse, rat
• Clonality: Polyclonal
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Positions: 143-170
• Sequence Length: 264

• Applicable Applications for anti-ALYREF antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~ 1:1000
• Antigen Type: Synthetic Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysate from KG-1 cell line,using THOC4 Antibody(MBS9211031). MBS9211031 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.)

Related Product Information for anti-ALYREF antibody

Export adapter involved in nuclear export of spliced and unspliced mRNA. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway). Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm. TREX recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production; ALYREF/THOC4 mediates the recruitment of the TREX complex to the intronless viral mRNA. Required for TREX complex assembly and for linking DDX39B to the cap-binding complex (CBC). In conjunction with THOC5 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in the nuclear export of intronless mRNA; proposed to be recruited to intronless mRNA by ATP-bound DDX39B. Involved in transcription elongation and genome stability.

References

Zody M.C.,et al.Nature 440:1045-1049(2006).
Quadroni M.,et al.Submitted (OCT-2004) to UniProtKB.
Bienvenut W.V.,et al.Submitted (DEC-2008) to UniProtKB.
Wichmann I.,et al.Hum. Immunol. 60:57-62(1999).
Rappsilber J.,et al.Genome Res. 12:1231-1245(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 238776833
• NCBI GeneID: 10189
• NCBI Accession #: NP_005773.3
• NCBI GenBank Nucleotide #: NM_005782.3
• UniProt Accession #: Q86V81; O43672
• Molecular Weight: 26888
• NCBI Official Full Name: THO complex subunit 4
• NCBI Official Synonym Full Names: Aly/REF export factor
• NCBI Official Symbol: ALYREF
• NCBI Official Synonym Symbols: ALY; BEF; REF; THOC4; ALY/REF
• NCBI Protein Information: THO complex subunit 4
• UniProt Protein Name: THO complex subunit 4
• Protein Family: THO complex
• UniProt Gene Name: ALYREF
• UniProt Synonym Gene Names: ALY; BEF; THOC4; Tho4
• UniProt Entry Name: THOC4_HUMAN

NCBI Description

The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

THOC4: a chaperone protein that promotes the dimerization of transcription factors containing basic leucine zipper (bZIP) domains thus promoting transcriptional activation. Plays a role in mRNA processing and export. May function as scaffold that mediates interactions between proteins and/or RNA. Integral part of the THO/TREX complex that is recruited to transcribed genes and travels with the RNA polymerase during elongation. Is part of the exon junction complex that remains associated with spliced mRNA and plays an important role in mRNA export and nonsense-mediated RNA decay. Nuclear localization, translocates to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Two differentially spliced isoforms have been described.

Protein type: Transcription, coactivator/corepressor; Chaperone; RNA-binding; Spliceosome

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: nucleoplasm; membrane; intracellular membrane-bound organelle; nuclear speck; cytosol; nucleus

Molecular Function: protein binding; nucleotide binding; single-stranded DNA binding

Biological Process: osteoblast differentiation; nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; mRNA export from nucleus; positive regulation of RNA elongation; RNA splicing; regulation of DNA recombination; gene expression; intronless viral mRNA export from host nucleus; mRNA 3'-end processing; termination of RNA polymerase II transcription; replication fork processing