Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CNOT10 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Rabbit anti-Human, Mouse CNOT10 Polyclonal Antibody | anti-CNOT10 antibody
CNOT10 Polyclonal Antibody
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity Purification
Synonyms
CNOT10; Polyclonal Antibody; CNOT10 Polyclonal Antibody; anti-CNOT10 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence Length
744
Applicable Applications for anti-CNOT10 antibody
Western Blot (WB)
Application Notes
WB: 1:500 - 1:2000
Immunogen
Recombinant protein of human CNOT10
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CNOT10 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CNOT10 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Product Categories/Family for anti-CNOT10 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
82kDa
NCBI Official Full Name
CCR4-NOT transcription complex subunit 10
NCBI Official Synonym Full Names
CCR4-NOT transcription complex subunit 10
NCBI Official Symbol
CNOT10
NCBI Protein Information
CCR4-NOT transcription complex subunit 10
UniProt Protein Name
CCR4-NOT transcription complex subunit 10
Protein Family
UniProt Gene Name
CNOT10
Uniprot Description
CNOT10: Belongs to the CNOT10 family. 5 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 3p22.3
Cellular Component: CCR4-NOT complex; cytosol; membrane
Molecular Function: protein binding
Biological Process: DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; poly(A) tail shortening
Research Articles on CNOT10
Similar Products
Product Notes
The CNOT10 cnot10 (Catalog #AAA9132076) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CNOT10 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CNOT10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the CNOT10 cnot10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CNOT10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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