NAA10/ARD1 Recombinant Protein | NAA10 recombinant protein

NAA10/ARD1 Protein, Human, Recombinant

Cat. Num. AAA8121662

• Gene Names: NAA10; TE2; ARD1; NATD; ARD1A; ARD1P; DXS707; MCOPS1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: NAA10/ARD1; NAA10/ARD1 Protein; Human; Recombinant; Human NAA10/ARD1A Protein; ARD1 Protein; ARD1A Protein; ARD1P Protein; DXS707 Protein; MCOPS1 Protein; NAA10 Protein; NATD Protein; OGDNS Protein; TE2 Protein; N(alpha)-acetyltransferase 10; NatA catalytic subunit; NAA10 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser235

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gly
• Tag: N-cleavage
• Protein Construction: A DNA sequence encoding the human ARD1A (P41227)(Met1-Ser 235) was expressed and purified.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for NAA10 recombinant protein

Background: ARD1 is a member of the 2-kDa ARF protein family. It is a multifunctional protein. ARD1 has an 18-kDa ADP-ribosylation factor (ARF) domain at the C-terminus (amino acids 43-574), and a 46-kDa N-terminal domain (amino acids 1-42). The C-terminal region of ARD1 may be involved in the formation of both ARD1-ARD1 and ARD1-NAT1 complexes. ARD1 and NAT1 genes are required for the expression of an N-terminal protein acetyltransferase. This activity is required for full repression of the silent mating-type locus HML, for sporulation, and for entry into G. Recombinant ARD1 (amino acids 1-574) or its RING finger domain (amino acids 1-11) produced polyubiquitylated proteins when incubated in vitro with a mammalian E1, an E2 enzyme, ATP, and ubiquitin.

References

Tribioli C., et al.,(1994), Isolation of new genes in distal Xq28: transcriptional map and identification of a human homologue of the ARD1 N-acetyl transferase of Saccharomyces cerevisiae. Hum. Mol. Genet. 3:1061-1068.Arnesen T., et al., (2005), Identification and characterization of the human ARD1-NATH protein acetyltransferase complex.Biochem. J. 386:433-443.Ross M.T., et al.,(2005), The DNA sequence of the human X chromosome.Nature 434:325-337.

NCBI and Uniprot Product Information

• NCBI GI #: 728880
• NCBI GeneID: 8260
• UniProt Accession #: P41227; A6NM98
• Molecular Weight: 235
• NCBI Official Full Name: N-alpha-acetyltransferase 10
• NCBI Official Synonym Full Names: N(alpha)-acetyltransferase 10, NatA catalytic subunit
• NCBI Official Symbol: NAA10
• NCBI Official Synonym Symbols: TE2; ARD1; NATD; ARD1A; ARD1P; DXS707; MCOPS1
• NCBI Protein Information: N-alpha-acetyltransferase 10; natA catalytic subunit Naa10; ARD1 homolog A, N-acetyltransferase; N-acetyltransferase ARD1, human homolog of; N-alpha-acetyltransferase 10, NatA catalytic subunit; N-terminal acetyltransferase complex ARD1 subunit homolog A
• UniProt Protein Name: N-alpha-acetyltransferase 10
• Protein Family: N-alpha-acetyltransferase
• UniProt Gene Name: NAA10
• UniProt Synonym Gene Names: ARD1; ARD1A; TE2
• UniProt Entry Name: NAA10_HUMAN

NCBI Description

N-alpha-acetylation is among the most common post-translational protein modifications in eukaryotic cells. This process involves the transfer of an acetyl group from acetyl-coenzyme A to the alpha-amino group on a nascent polypeptide and is essential for normal cell function. This gene encodes an N-terminal acetyltransferase that functions as the catalytic subunit of the major amino-terminal acetyltransferase A complex. Mutations in this gene are the cause of Ogden syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

NAA10: In complex with NAA15, displays alpha (N-terminal) acetyltransferase activity. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation. Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration. Interacts with HIF1A (via its ODD domain); the interaction increases HIF1A protein stability during normoxia, and down-regulates it when induced by hypoxia. Interacts with NAA15, NAA50 and with the ribosome. Binds to MYLK. Ubiquitous. Belongs to the acetyltransferase family. ARD1 subfamily.

Protein type: Amino Acid Metabolism - tyrosine; Secondary Metabolites Metabolism - limonene and pinene degradation; Amino Acid Metabolism - phenylalanine; EC 2.3.1.88; Lipid Metabolism - glycerophospholipid; Acetyltransferase

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: membrane; cytoplasm; nucleolus; intracellular; nucleus

Molecular Function: peptide alpha-N-acetyltransferase activity; N-acetyltransferase activity; protein binding; acetyltransferase activity; ribosome binding

Biological Process: N-terminal protein amino acid acetylation; N-terminal peptidyl-glutamic acid acetylation; internal protein amino acid acetylation; N-terminal peptidyl-serine acetylation; DNA packaging

Disease: Ogden Syndrome; Microphthalmia, Syndromic 1