Western Blot (WB)
(Western blot analysis of TGIF1 (arrow) using rabbit polyclonal TGIF1 Antibody (Center L223) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TGIF1 gene.)
Rabbit anti-Human, Mouse TGIF1 Polyclonal Antibody
TGIF1, ID (L223) (TGIF1, TGIF, Homeobox protein TGIF1, 5'-TG-3'-interacting factor 1)
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
PurifiedPurified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Synonyms
TGIF1; Polyclonal Antibody; ID (L223) (TGIF1; TGIF; Homeobox protein TGIF1; 5'-TG-3'-interacting factor 1); Anti -TGIF1; anti-TGIF1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, mouse
Purity/Purification
Purified
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-TGIF1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:50-100
Dilution: ELISA: 1:1,000
Western Blot: 1:50-100
Immunogen
TGIF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 215-245 amino acids from the Central region of human TGIF1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of TGIF1 (arrow) using rabbit polyclonal TGIF1 Antibody (Center L223) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TGIF1 gene.)
Western Blot (WB)
(Western blot analysis of TGIF1 (arrow) using rabbit polyclonal TGIF1 Antibody (Center L223) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TGIF1 gene.)
Western Blot (WB)
(TGIF1 Antibody (Center L223) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the TGIF1 antibody detected the TGIF1 protein (arrow).)
Western Blot (WB)
(TGIF1 Antibody (Center L223) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the TGIF1 antibody detected the TGIF1 protein (arrow).)
Related Product Information for anti-TGIF1 antibody
The protein encoded by this gene is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4, which is a structural anomaly of the brain. Alternative splicing has been observed at this locus and eight variants, encoding four distinct isoforms, are described.
Product Categories/Family for anti-TGIF1 antibody
Similar Products
Product Notes
The TGIF1 (Catalog #AAA645263) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TGIF1, ID (L223) (TGIF1, TGIF, Homeobox protein TGIF1, 5'-TG-3'-interacting factor 1) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TGIF1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:50-100. Researchers should empirically determine the suitability of the TGIF1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TGIF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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