Rabbit anti-Human, Mouse TGIF1 Polyclonal Antibody | anti-TGIF1 antibody

TGIF1, ID (L223) (TGIF1, TGIF, Homeobox protein TGIF1, 5'-TG-3'-interacting factor 1) (AP)

Cat. Num. AAA6355239

• Gene Names: TGIF1; HPE4; TGIF
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: TGIF1; Polyclonal Antibody; ID (L223) (TGIF1; TGIF; Homeobox protein TGIF1; 5'-TG-3'-interacting factor 1) (AP); 5'-TG-3'-interacting factor 1; anti-TGIF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
• Sequence Length: 272

• Applicable Applications for anti-TGIF1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TGIF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 215-245 amino acids from the Central region of human TGIF1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of TGIF1 (arrow) using rabbit polyclonal TGIF1 Antibody (Center L223). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TGIF1 gene.)

Western Blot (WB)

(TGIF1 Antibody (Center L223) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the TGIF1 antibody detected the TGIF1 protein (arrow).)

Related Product Information for anti-TGIF1 antibody

The protein encoded by this gene is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4, which is a structural anomaly of the brain. Alternative splicing has been observed at this locus and eight variants, encoding four distinct isoforms, are described.

Product Categories/Family for anti-TGIF1 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

References

Bengoechea-Alonso, M.T., et al. Oncogene 29(38):5322-5328(2010). Paulussen, A.D., et al. Eur. J. Hum. Genet. 18(9):999-1005(2010). Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010): Demange, C., et al. Mol. Cell 36(6):1073-1085(2009). Hamid, R., et al. Mol Oncol 3 (5-6), 451-463 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4507473
• NCBI GeneID: 7050
• NCBI Accession #: NP_003235.1; NP_733796.2
• NCBI GenBank Nucleotide #: NP_003235.1
• UniProt Accession #: Q15583
• NCBI Official Full Name: homeobox protein TGIF1 isoform c
• NCBI Official Synonym Full Names: TGFB induced factor homeobox 1
• NCBI Official Symbol: TGIF1
• NCBI Official Synonym Symbols: HPE4; TGIF
• NCBI Protein Information: homeobox protein TGIF1
• UniProt Protein Name: Homeobox protein TGIF1
• Protein Family: Homeobox protein
• UniProt Gene Name: TGIF1
• UniProt Synonym Gene Names: TGIF
• UniProt Entry Name: TGIF1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4, which is a structural anomaly of the brain. Alternative splicing has been observed at this locus and multiple splice variants encoding distinct isoforms are described. [provided by RefSeq, Jul 2013]