Western Blot (WB)
((0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat TGFBI Polyclonal Antibody | anti-TGFBI antibody
Goat anti-TGFBI Antibody
Immunohistochemistry: In paraffin embedded Kidney shows staining of intercalated cells of a collecting tubule. Recommended concentration, 4-6ug/ml.
Western Blot: Approx 70kDa band observed in Human, Mouse and Rat Kidney lysates (calculated MW of 74.7kDa according to Human NP_000349.1). Recommended concentration: 0.2-0.5ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq, Jul 2008]
Uniprot Description
betaIG-H3: Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation. By TGFB1. Highly expressed in the corneal epithelium.
Protein type: Extracellular matrix; Cell adhesion; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 5q31
Cellular Component: basement membrane; extracellular matrix; extracellular region; extracellular space; plasma membrane; proteinaceous extracellular matrix; trans-Golgi network
Molecular Function: collagen binding; extracellular matrix binding; integrin binding; protein binding
Biological Process: angiogenesis; cell adhesion; cell proliferation; cellular protein metabolic process; chondrocyte differentiation; extracellular matrix organization and biogenesis; negative regulation of cell adhesion; response to stimulus; visual perception
Disease: Corneal Dystrophy Of Bowman Layer, Type I; Corneal Dystrophy Of Bowman Layer, Type Ii; Corneal Dystrophy, Avellino Type; Corneal Dystrophy, Epithelial Basement Membrane; Corneal Dystrophy, Groenouw Type I; Corneal Dystrophy, Lattice Type I; Corneal Dystrophy, Lattice Type Iiia
Research Articles on TGFBI
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Product Notes
The TGFBI tgfbi (Catalog #AAA421749) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-TGFBI Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's TGFBI can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Kidney shows staining of intercalated cells of a collecting tubule. Recommended concentration, 4-6ug/ml. Western Blot: Approx 70kDa band observed in Human, Mouse and Rat Kidney lysates (calculated MW of 74.7kDa according to Human NP_000349.1). Recommended concentration: 0.2-0.5ug/ml. Researchers should empirically determine the suitability of the TGFBI tgfbi for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QLYTDRTEKL RPE. It is sometimes possible for the material contained within the vial of "TGFBI, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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