Mouse anti-Mouse, Human VCP Antibody | anti-VCP antibody

Anti-VCP Purified

Cat. Num. AAA1800764

• Gene Names: VCP; p97; TERA; CDC48
• Reactivity: Mouse, Human
• Applications: Flow Cytometry, Functional Assay, Western Blot, Immunocytochemistry
• Purity: Purified by Precipitation and Chromatography
• Synonyms: VCP; Anti-VCP Purified; valosin containing protein; TERA; CDC48; anti-VCP antibody

• Host: Mouse
• Reactivity: Mouse, Human
• Isotype: IgM
• Clone Number: Hs-14
• Specificity: The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa intra-acrosomal protein.
• Purity/Purification: Purified by Precipitation and Chromatography
• Form/Format: Tris buffered saline (TBS) solution with 15 mM sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 806

• Applicable Applications for anti-VCP antibody: Flow Cytometry (FC/FACS), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: FC/FACS: QC tested
• Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
• Preparation and Storage: Store at 2-8 degree C.

Immunocytochemistry (ICC)

(Immunocytochemistry analysis of VCP in acetone-permeabilized human sperms (normal spermiogram)using monoclonal antibody Hs-14 demonstrates its location to the acrosome.)

Related Product Information for anti-VCP antibody

VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.

NCBI and Uniprot Product Information

• NCBI GI #: 6005942
• NCBI GeneID: 7415
• NCBI Accession #: NP_009057.1
• NCBI GenBank Nucleotide #: NP_009057.1
• UniProt Accession #: P55072
• NCBI Official Full Name: transitional endoplasmic reticulum ATPase isoform 1
• NCBI Official Synonym Full Names: valosin containing protein
• NCBI Official Symbol: VCP
• NCBI Official Synonym Symbols: p97; TERA; CDC48
• NCBI Protein Information: transitional endoplasmic reticulum ATPase
• UniProt Protein Name: Transitional endoplasmic reticulum ATPase
• Protein Family: VCP-like ATPase
• UniProt Gene Name: VCP
• UniProt Synonym Gene Names: TER ATPase; VCP
• UniProt Entry Name: TERA_HUMAN

NCBI Description

This gene encodes a member of the AAA ATPase family of proteins. The encoded protein plays a role in protein degradation, intracellular membrane fusion, DNA repair and replication, regulation of the cell cycle, and activation of the NF-kappa B pathway. This protein forms a homohexameric complex that interacts with a variety of cofactors and extracts ubiquitinated proteins from lipid membranes or protein complexes. Mutations in this gene cause IBMPFD (inclusion body myopathy with paget disease of bone and frontotemporal dementia), ALS (amyotrophic lateral sclerosis) and Charcot-Marie-Tooth disease in human patients. [provided by RefSeq, Aug 2017]

Uniprot Description

VCP: valosin-containing protein (VCP) is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the nuclear envelope and of the transitional endoplasmic reticulum (tER). Regulates NFKappaB pathway, which is important for metastasis of osteosarcoma. Tyrosine phosphorylation regulates its cell cycle-dependent nuclear localization.

Protein type: Chaperone; DNA repair, damage; Endoplasmic reticulum; Hydrolase; EC 3.6.4.6

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: proteasome complex; nucleoplasm; endoplasmic reticulum membrane; intracellular membrane-bound organelle; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; lipid particle; cytosol; nucleus

Molecular Function: protein domain specific binding; identical protein binding; protein binding; ATPase activity; protein complex binding; ADP binding; polyubiquitin binding; protein phosphatase binding; lipid binding; ATP binding; receptor binding

Biological Process: caspase activation; proteasomal ubiquitin-dependent protein catabolic process; ER to Golgi vesicle-mediated transport; ER-associated protein catabolic process; unfolded protein response; protein ubiquitination; DNA repair; retrograde protein transport, ER to cytosol; regulation of apoptosis; bypass DNA synthesis; establishment of protein localization; positive regulation of protein complex assembly; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; double-strand break repair; positive regulation of protein catabolic process; protein amino acid N-linked glycosylation via asparagine; response to DNA damage stimulus; protein homooligomerization

Disease: Inclusion Body Myopathy With Early-onset Paget Disease With Or Without Frontotemporal Dementia 1; Amyotrophic Lateral Sclerosis 14, With Or Without Frontotemporal Dementia