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Testing Data (Anti-GDF8 Antibody at 1:2000 dilution + mouse liver lysatennLysates/proteins at 20 u03bcg per lane. nnSecondarynGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. nnPredicted band size : 43 kDannBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human, Mouse Myostatin Polyclonal Antibody | anti-Myostatin antibody

Myostatin (N-term) Rabbit pAb

Gene Names
MSTN; GDF8; MSLHP
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
Myostatin; Polyclonal Antibody; Myostatin (N-term) Rabbit pAb; Growth/differentiation factor 8; GDF-8; MSTN; GDF8; anti-Myostatin antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Purity/Purification
Affinity purified
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pHbuffers and neutralized immediately, followed by dialysis against PBS.
Applicable Applications for anti-Myostatin antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:2000
IHC: 1:50
Immunogen
This Myostatin (GDF8) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-38 amino acids from the N-terminal region of human Myostatin (GDF8).
Conjugation
Unconjugated
Cell Position
Secreted

Testing Data

(Anti-GDF8 Antibody at 1:2000 dilution + mouse liver lysatennLysates/proteins at 20 u03bcg per lane. nnSecondarynGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. nnPredicted band size : 43 kDannBlocking/Dilution buffer: 5% NFDM/TBST.)

Testing Data (Anti-GDF8 Antibody at 1:2000 dilution + mouse liver lysatennLysates/proteins at 20 u03bcg per lane. nnSecondarynGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. nnPredicted band size : 43 kDannBlocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with GDF8 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with GDF8 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for)

Testing Data

(Anti-GDF8 Antibody (N24) at 1:2000 dilution + human skeletal muscle lysatennLysates/proteins at 20 u03bcg per lane. nnSecondarynGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. nnPredicted band size : 43 kDannBlocking/Dilution buffer: 5% NFDM/TBST.)

Testing Data (Anti-GDF8 Antibody (N24) at 1:2000 dilution + human skeletal muscle lysatennLysates/proteins at 20 u03bcg per lane. nnSecondarynGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. nnPredicted band size : 43 kDannBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-Myostatin antibody
GDF8 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. This gene is thought to encode a secreted protein which negatively regulates skeletal muscle growth.
Product Categories/Family for anti-Myostatin antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42,750 Da
NCBI Official Full Name
growth/differentiation factor 8 preproprotein
NCBI Official Synonym Full Names
myostatin
NCBI Official Symbol
MSTN
NCBI Official Synonym Symbols
GDF8; MSLHP
NCBI Protein Information
growth/differentiation factor 8
UniProt Protein Name
Growth/differentiation factor 8
UniProt Gene Name
MSTN
UniProt Synonym Gene Names
GDF8; GDF-8
UniProt Entry Name
GDF8_HUMAN

Uniprot Description

MSTN: Acts specifically as a negative regulator of skeletal muscle growth. Defects in MSTN are the cause of muscle hypertrophy (MSLHP). MSLHP is a condition characterized by increased muscle bulk and strength. Affected individuals are exceptionally strong. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2q32.2

Cellular Component: extracellular space

Molecular Function: cytokine activity; identical protein binding; protein binding; receptor binding; transforming growth factor beta receptor binding

Biological Process: cell development; muscle development; muscle maintenance; negative regulation of myoblast differentiation; negative regulation of protein kinase B signaling cascade; negative regulation of skeletal muscle growth; positive regulation of transcription, DNA-dependent; regulation of apoptosis; regulation of MAPKKK cascade

Disease: Muscle Hypertrophy

Similar Products

Product Notes

The Myostatin mstn (Catalog #AAA4757726) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Myostatin (N-term) Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Myostatin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:2000 IHC: 1:50. Researchers should empirically determine the suitability of the Myostatin mstn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myostatin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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