Rabbit anti-Mouse Fas Ligand Polyclonal Antibody | anti-FASLG antibody

Anti-Fas Ligand Antibody, Rabbit Polyclonal

Cat. Num. AAA8101620

• Gene Names: Fasl; gld; CD178; CD95L; Fas-L; Faslg; CD95-L; Tnfsf6; APT1LG1
• Reactivity: Mouse
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: Fas Ligand; Polyclonal Antibody; Anti-Fas Ligand Antibody; Rabbit Polyclonal; Fas Ligand/FASLG/CD95L Antibody; Rabbit PAb; Antigen Affinity Purified; Fas ligand; Anti-Apt1lg1 Antibody; Anti-CD178 Antibody; Anti-CD95-L Antibody; Anti-Cd95l Antibody; Anti-Fas-L Antibody; Anti-Fasl Antibody; Anti-Faslg Antibody; Anti-gld Antibody; Anti-Tnfsf6 Antibody; anti-FASLG antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse Fas Ligand/FASLG/CD95L
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-FASLG antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:500-1:2000; ICC/IF: 1:300-1:10,000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Mouse Fas Ligand/FASLG/CD95L
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Mouse Fas Ligand/FASLG/CD95L, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of mouse Faslg in mouse small intestine with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunofluorescence (IF)

(Immunofluorescence staining of FASLG in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human FASLG polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.)

Related Product Information for anti-FASLG antibody

Fas Ligand, also known as FASLG and CD95L, is the ligand for FAS. It is a transmembrane protein which binds to TNFRSF6/FAS. Interaction of FAS with fas Ligand is critical in triggering apoptosis of some types of cells such as lymphocytes. Fas Ligand may be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.

Product Categories/Family for anti-FASLG antibody

Death Receptor Signaling

References

Pitti R M. et al., 1998, Nature. 396 (6712): 699-703. Hane M. et al., 1995, FEBS Lett. 373 (3): 265-8. Schneider P. et al., 1997, J Biol Chem. 272 (30): 18827-33.

NCBI and Uniprot Product Information

• NCBI GI #: 729462
• NCBI GeneID: 14103
• UniProt Accession #: P41047; Q61217; Q9R1F2
• Molecular Weight: 8,040 Da
• NCBI Official Full Name: Tumor necrosis factor ligand superfamily member 6
• NCBI Official Synonym Full Names: Fas ligand (TNF superfamily, member 6)
• NCBI Official Symbol: Fasl
• NCBI Official Synonym Symbols: gld; CD178; CD95L; Fas-L; Faslg; CD95-L; Tnfsf6; APT1LG1
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 6; CD95 ligand; Fas antigen ligand; generalized lymphoproliferative disease
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 6
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: Faslg
• UniProt Synonym Gene Names: Apt1lg1; Cd95l; Fasl; gld; Tnfsf6; CD95-L; Fas ligand; FasL; sFasL; APL; FasL ICD; SPA
• UniProt Entry Name: TNFL6_MOUSE

Uniprot Description

FasL: Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. Homotrimer (Probable). Interacts with ARHGAP9, BAIAP2L1, BTK, CACNB3, CACNB4, CRK, DLG2, DNMBP, DOCK4, EPS8L3, FGR, FYB, FYN, HCK, ITK, ITSN2, KALRN, LYN, MACC1, MIA, MPP4, MYO15A, NCF1, NCK1, NCK2, NCKIPSD, OSTF1, PIK3R1, PSTPIP1, RIMBP3C, SAMSN1, SH3GL3, SH3PXD2B, SH3PXD2A, SH3RF2, SKAP2, SNX33, SNX9, SORBS3, SPTA1, SRC, SRGAP1, SRGAP2, SRGAP3, TEC, TJP3 and YES1. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Cytokine; Membrane protein, integral

Cellular Component: extracellular space; lysosome; cytoplasmic membrane-bound vesicle; integral to membrane; extracellular region; caveola; lipid raft; membrane; perinuclear region of cytoplasm; plasma membrane; cytoplasmic vesicle; nucleus; external side of plasma membrane

Molecular Function: death receptor binding; cytokine activity; tumor necrosis factor receptor binding

Biological Process: caspase activation; transcription, DNA-dependent; positive regulation of apoptosis; apoptosis; negative regulation of transcription from RNA polymerase II promoter; cellular chloride ion homeostasis; endosomal lumen acidification; inflammatory cell apoptosis; negative regulation of angiogenesis; regulation of transcription, DNA-dependent; induction of apoptosis via death domain receptors; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of neuron apoptosis; positive regulation of cell proliferation; immune response; retinal cell programmed cell death