Western Blot (WB)
(Anti-TFE3 Antibody (N-term)at 1:2000 dilution + HepG2 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 62 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human, mouse TFE3 Polyclonal Antibody | anti-TFE3 antibody
TFE3 Antibody (N-term)
IF~~1:10~50
Western Blot (WB)
(Anti-TFE3 Antibody (N-term)at 1:2000 dilution + HepG2 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 62 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-TFE3 Antibody (N-term)at 1:2000 dilution + HepG2 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 62 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(TFE3 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TFE3 Antibody detected the TFE3 protein (arrow).)
Western Blot (WB)
(TFE3 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TFE3 Antibody detected the TFE3 protein (arrow).)
Western Blot (WB)
(TFE3 Antibody (N-term) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the TFE3 antibody detected the TFE3 protein (arrow).)
Western Blot (WB)
(TFE3 Antibody (N-term) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the TFE3 antibody detected the TFE3 protein (arrow).)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with TFE3 Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFE3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TFE3 immunoreactivity is localized to nucleus and Cytoplasm significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with TFE3 Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFE3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TFE3 immunoreactivity is localized to nucleus and Cytoplasm significantly.)
factor E (MITF-TFE) family of basic helix-loop-helix leucine zipper
(bHLH-Zip) transcription factors includes four family members:
MITF, TFE3, TFEB and TFEC. The TEF3 protein encoded by this gene
activates transcription through binding to the muE3 motif of the
immunoglobulin heavy-chain enhancer. The TFEC protein forms
heterodimers with the TEF3 protein and inhibits TFE3-dependent
transcription activation. The TEF3 protein interacts with
transcription regulators such as E2F3, SMAD3, and LEF-1, and is
involved in TGF-beta-induced transcription, playing important roles
in cell growth, proliferation, and osteoclast and macrophage
differentiation. The TFE3 protein also activates hepatic IRS-2
gene, and induces hexokinase II (HK2) and insulin-induced gene 1
(INSIG1); it participates in insulin signaling and could be a
therapeutic target for diabetes. This gene is also involved in
chromosomal translocations, resulting in different fusion gene
products in papillary renal cell carcinomas and alveolar soft part
sarcomas, such as PRCC-TFE3, RCC17-TFE3, PSF-TFE3, NonO
(p54nrb)-TFE3 and ASPL-TFE3.
Haudebourg, J., et al. Cancer Genet. Cytogenet. 200(2):75-78(2010)
Chang, I.W., et al. Am. J. Surg. Pathol. 33(12):1894-1901(2009)
Yamaguchi, T., et al. Acta Cytol. 53(6):693-697(2009)
Kuroda, N., et al. Pathol. Int. 59(10):769-770(2009)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a basic helix-loop-helix domain-containing transcription factor that binds MUE3-type E-box sequences in the promoter of genes. The encoded protein promotes the expression of genes downstream of transforming growth factor beta (TGF-beta) signaling. This gene may be involved in chromosomal translocations in renal cell carcinomas and other cancers, resulting in the production of fusion proteins. Translocation partners include PRCC (papillary renal cell carcinoma), NONO (non-POU domain containing, octamer-binding), and ASPSCR1 (alveolar soft part sarcoma chromosome region, candidate 1), among other genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
Uniprot Description
TFE3: Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site. A chromosomal aberration involving TFE3 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein. Chromosomal aberrations involving TFE3 are found in patients with papillary renal cell carcinoma. Translocation t(X;1)(p11.2;q21.2) with PRCC; translocation t(X;1)(p11.2;p34) with PSF; inversion inv(X)(p11.2;q12) that fuses NONO to TFE3. Belongs to the MiT/TFE family.
Protein type: Transcription factor; Oncoprotein; DNA-binding
Chromosomal Location of Human Ortholog: Xp11.22
Cellular Component: nucleoplasm; cytoplasm; nucleus
Molecular Function: protein dimerization activity; transcription factor activity
Biological Process: regulation of osteoclast differentiation; positive regulation of cell adhesion; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; humoral immune response
Disease: Renal Cell Carcinoma, Xp11-associated
Research Articles on TFE3
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Product Notes
The TFE3 tfe3 (Catalog #AAA9211960) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The TFE3 Antibody (N-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TFE3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF). WB~~1:1000 IF~~1:10~50. Researchers should empirically determine the suitability of the TFE3 tfe3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TFE3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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