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Western Blot (WB) (Western blot analysis of anti-TESK2 Antibody (Center) in HL60 cell line lysates (35ug/lane). TESK2 (arrow) was detected using the purified Pab.)

Rabbit TESK2 Polyclonal Antibody | anti-TESK2 antibody

TESK2 Antibody (Center C225)

Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TESK2; Polyclonal Antibody; TESK2 Antibody (Center C225); Dual specificity testis-specific protein kinase 2; Testicular protein kinase 2; anti-TESK2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TESK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 210-240 amino acids from the Central region of human TESK2.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
210-240
Sequence Length
571
Applicable Applications for anti-TESK2 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of anti-TESK2 Antibody (Center) in HL60 cell line lysates (35ug/lane). TESK2 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of anti-TESK2 Antibody (Center) in HL60 cell line lysates (35ug/lane). TESK2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with TESK2 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human testis tissue reacted with TESK2 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Related Product Information for anti-TESK2 antibody
TESK2 is a dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. TESK2 phosphorylates cofilin at Ser-3. It may play an important role in spermatogenesis. This nuclear enzyme is thought to be activated by autophosphorylation on Ser-219. Expression is predominantly in testis and prostate, with highest concentrations in nongerminal Sertoli cells.
Product Categories/Family for anti-TESK2 antibody
References
Toshima,J.,J. Biol. Chem. 276 (33), 31449-31458 (2001)
Rosok,O., Genomics 61 (1), 44-54 (1999)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
63639
NCBI Official Full Name
dual specificity testis-specific protein kinase 2
NCBI Official Synonym Full Names
testis-specific kinase 2
NCBI Official Symbol
TESK2
NCBI Protein Information
dual specificity testis-specific protein kinase 2
UniProt Protein Name
Dual specificity testis-specific protein kinase 2
UniProt Gene Name
TESK2
UniProt Entry Name
TESK2_HUMAN

NCBI Description

This gene product is a serine/threonine protein kinase that contains an N-terminal protein kinase domain that is structurally similar to the kinase domains of testis-specific protein kinase-1 and the LIM motif-containing protein kinases (LIMKs). Its overall structure is most related to the former, indicating that it belongs to the TESK subgroup of the LIMK/TESK family of protein kinases. This gene is predominantly expressed in testis and prostate. The developmental expression pattern of the rat gene in testis suggests an important role for this gene in meitoic stages and/or early stages of spermiogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

TESK2: a TKL kinase of the LISK family. Predominantly expressed in testis and prostate. The developmental expression pattern in the testis suggests an important role in meitoic stages and/or early stages of spermiogenesis. Three alternatively spliced isoforms have been described.

Protein type: Protein kinase, TKL; Kinase, protein; EC 2.7.12.1; Protein kinase, Ser/Thr (non-receptor); TKL group; LISK family; TESK subfamily

Chromosomal Location of Human Ortholog: 1p32

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; metal ion binding; protein serine/threonine/tyrosine kinase activity; ATP binding; protein kinase activity

Biological Process: focal adhesion formation; peptidyl-tyrosine phosphorylation; spermatogenesis; actin cytoskeleton organization and biogenesis; protein amino acid phosphorylation

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Product Notes

The TESK2 tesk2 (Catalog #AAA9205571) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 210-240. The TESK2 Antibody (Center C225) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's TESK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the TESK2 tesk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TESK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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