Mouse DDX50 Monoclonal Antibody | anti-DDX50 antibody

DDX50 (DEAD (Asp-Glu-Ala-Asp) Box 50, GU2, GUB, MGC3199, RH-II/GuB)

Cat. Num. AAA601367

• Gene Names: DDX50; GU2; GUB; MGC3199; RH-II/GuB
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: DDX50; Monoclonal Antibody; DDX50 (DEAD (Asp-Glu-Ala-Asp) Box 50; GU2; GUB; MGC3199; RH-II/GuB); Anti -DDX50 (DEAD (Asp-Glu-Ala-Asp) Box 50; anti-DDX50 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8C108
• Specificity: Recognizes human DDX50.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

• Applicable Applications for anti-DDX50 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blotting.; Dilution: Western Blotting: 1:1000-1:2000
• Immunogen: Partial sequence of recombinant full-length protein to human DDX50
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

Product Categories/Family for anti-DDX50 antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 39644658
• NCBI GeneID: 79009
• UniProt Accession #: Q53FI9; Q9BQ39; Q9NTA9
• Molecular Weight: 41,296 Da
• NCBI Official Full Name: DDX50 protein
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box polypeptide 50
• NCBI Official Symbol: DDX50
• NCBI Official Synonym Symbols: GU2; GUB; MGC3199; RH-II/GuB
• NCBI Protein Information: ATP-dependent RNA helicase DDX50; gu-beta; OTTHUMP00000019711; DEAD box protein 50; nucleolar protein GU2; RNA helicase II/Gu beta
• UniProt Protein Name: Nucleolar protein GU2 variant
• Protein Family: ATP-dependent RNA helicase
• UniProt Entry Name: Q53FI9_HUMAN

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. This gene and DDX21, also called RH-II/GuA, have similar genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution. This gene has pseudogenes on chromosomes 2, 3 and 4. Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined. [provided by RefSeq]

Uniprot Description

DDX50: an RNA helicase of the DEAD-box family.

Protein type: Nucleolus; RNA-binding; Helicase; EC 3.6.4.13

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: membrane; nucleolus; plasma membrane

Molecular Function: helicase activity; ATP binding

Biological Process: metabolic process