Rabbit anti-Human, Mouse TERF2 Polyclonal Antibody | anti-TERF2 antibody

TERF2 (Telomeric Repeat Binding Factor 2, TRF2, TRBF2) (MaxLight 650)

Cat. Num. AAA6440276

• Gene Names: TERF2; TRF2; TRBF2
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: TERF2; Polyclonal Antibody; TERF2 (Telomeric Repeat Binding Factor 2; TRF2; TRBF2) (MaxLight 650); anti-TERF2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes TERF2. Species Crossreactivity: human, mouse
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-TERF2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human TERF2.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Product Categories/Family for anti-TERF2 antibody

Antibodies; Telomere/Telomerase

NCBI and Uniprot Product Information

• NCBI GI #: 613504836
• NCBI GeneID: 7014
• UniProt Accession #: Q15554
• Molecular Weight: 500
• NCBI Official Full Name: Telomeric repeat-binding factor 2
• NCBI Official Synonym Full Names: telomeric repeat binding factor 2
• NCBI Official Symbol: TERF2
• NCBI Official Synonym Symbols: TRF2; TRBF2
• NCBI Protein Information: telomeric repeat-binding factor 2; telomeric DNA-binding protein; TTAGGG repeat-binding factor 2; telomeric repeat binding protein 2
• UniProt Protein Name: Telomeric repeat-binding factor 2
• Protein Family: Telomeric repeat-binding factor
• UniProt Gene Name: TERF2
• UniProt Synonym Gene Names: TRBF2; TRF2
• UniProt Entry Name: TERF2_HUMAN

NCBI Description

This gene encodes a telomere specific protein, TERF2, which is a component of the telomere nucleoprotein complex. This protein is present at telomeres in metaphase of the cell cycle, is a second negative regulator of telomere length and plays a key role in the protective activity of telomeres. While having similar telomere binding activity and domain organization, TERF2 differs from TERF1 in that its N terminus is basic rather than acidic. [provided by RefSeq, Jul 2008]

Uniprot Description

TRF2: Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single- stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology- directed repair (HDR), which can affect telomere length. Homodimer. Component of the shelterin complex (telosome) composed of TERF1, TERF2, TINF2, TERF2IP/RAP1, ACD and POT1. Interacts with TERF2IP. Interacts with NBN. Interacts with SLX4/BTBD12. Interacts with DCLRE1B/Apollo and TERF2IP/RAP1; the interaction is direct. Ubiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; DNA repair, damage

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: nucleoplasm; male germ cell nucleus; Golgi apparatus; chromosome, telomeric region; nuclear telomere cap complex; Mre11 complex; nuclear chromosome, telomeric region; cytoplasm; nucleus

Molecular Function: protein C-terminus binding; protein binding; protein homodimerization activity; telomeric DNA binding; double-stranded telomeric DNA binding; chromatin binding

Biological Process: negative regulation of telomere maintenance; telomere capping; negative regulation of telomere maintenance via semi-conservative replication; in utero embryonic development; telomeric loop formation; positive regulation of nitric-oxide synthase activity; positive regulation of telomere maintenance; telomere maintenance via telomerase; cell cycle; age-dependent telomere shortening; telomere maintenance; protection from non-homologous end joining at telomere