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SDS-Page

NPM2 recombinant protein

NPM2, 1-214aa, Human, His tag, E Coli

Applications
SDS-Page
Purity
> 85% by SDS-PAGE
Synonyms
NPM2; 1-214aa; Human; His tag; E Coli; nucleophosmin/ nucleoplasmin 2; NPM-2; NPM2 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 85% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 2mM DTT
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMNLSSAS STEEKAVTTV LWGCELSQER RTWTFRPQLE GKQSCRLLLH TICLGEKAKE EMHRVEILPP ANQEDKKMQP VTIASLQASV LPMVSMVGVQ LSPPVTFQLR AGSGPVFLSG QERYEASDLT WEEEEEEEGE EEEEEEEDDE DEDADISLEE QSPVKQVKRL VPQKQASVAK KKKLEKEEEE IRASVRDKSP VKKAKATARA KKPGFKK
Sequence Length
214
Applicable Applications for NPM2 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for NPM2 recombinant protein
NPM2, also known as nucleophosmin/nucleoplasmin 2, belongs to the nucleoplasmin family. This protein is core histones chaperone involved in chromatin reprogramming, especially during fertilization and early embryonic development. It probably involved in sperm DNA decondensation during fertilization. Recombinant human NPM2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for NPM2 recombinant protein
References
Platonova O., et al. (2011) Biochemistry 50:8078-8089

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26.6 kDa (237aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
NCBI Official Full Name
nucleoplasmin-2 isoform 1
NCBI Official Synonym Full Names
nucleophosmin/nucleoplasmin 2
NCBI Official Symbol
NPM2
NCBI Protein Information
nucleoplasmin-2
UniProt Protein Name
Nucleoplasmin-2
Protein Family
UniProt Gene Name
NPM2

Uniprot Description

Core histones chaperone involved in chromatin reprogramming, specially during fertilization and early embryonic development. Probably involved in sperm DNA decondensation during fertilization.

Research Articles on NPM2

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Product Notes

The NPM2 npm2 (Catalog #AAA204671) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's NPM2 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the NPM2 npm2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMNLSSAS STEEKAVTTV LWGCELSQER RTWTFRPQLE GKQSCRLLLH TICLGEKAKE EMHRVEILPP ANQEDKKMQP VTIASLQASV LPMVSMVGVQ LSPPVTFQLR AGSGPVFLSG QERYEASDLT WEEEEEEEGE EEEEEEEDDE DEDADISLEE QSPVKQVKRL VPQKQASVAK KKKLEKEEEE IRASVRDKSP VKKAKATARA KKPGFKK. It is sometimes possible for the material contained within the vial of "NPM2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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