Rabbit anti-Human, Mouse TERF2 Polyclonal Antibody | anti-TERF2 antibody

TERF2 (Telomeric Repeat Binding Factor 2, TRF2, TRBF2) (HRP)

Cat. Num. AAA6440272

• Gene Names: TERF2; TRF2; TRBF2
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: TERF2; Polyclonal Antibody; TERF2 (Telomeric Repeat Binding Factor 2; TRF2; TRBF2) (HRP); anti-TERF2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes TERF2. Species Crossreactivity: human, mouse
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-TERF2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human TERF2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of Jurkat cell line, using TERF2 antibody.)

Product Categories/Family for anti-TERF2 antibody

Antibodies; Telomere/Telomerase

NCBI and Uniprot Product Information

• NCBI GI #: 613504836
• NCBI GeneID: 7014
• UniProt Accession #: Q15554
• Molecular Weight: 500
• NCBI Official Full Name: Telomeric repeat-binding factor 2
• NCBI Official Synonym Full Names: telomeric repeat binding factor 2
• NCBI Official Symbol: TERF2
• NCBI Official Synonym Symbols: TRF2; TRBF2
• NCBI Protein Information: telomeric repeat-binding factor 2; telomeric DNA-binding protein; TTAGGG repeat-binding factor 2; telomeric repeat binding protein 2
• UniProt Protein Name: Telomeric repeat-binding factor 2
• Protein Family: Telomeric repeat-binding factor
• UniProt Gene Name: TERF2
• UniProt Synonym Gene Names: TRBF2; TRF2
• UniProt Entry Name: TERF2_HUMAN

NCBI Description

This gene encodes a telomere specific protein, TERF2, which is a component of the telomere nucleoprotein complex. This protein is present at telomeres in metaphase of the cell cycle, is a second negative regulator of telomere length and plays a key role in the protective activity of telomeres. While having similar telomere binding activity and domain organization, TERF2 differs from TERF1 in that its N terminus is basic rather than acidic. [provided by RefSeq, Jul 2008]

Uniprot Description

TRF2: Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single- stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology- directed repair (HDR), which can affect telomere length. Homodimer. Component of the shelterin complex (telosome) composed of TERF1, TERF2, TINF2, TERF2IP/RAP1, ACD and POT1. Interacts with TERF2IP. Interacts with NBN. Interacts with SLX4/BTBD12. Interacts with DCLRE1B/Apollo and TERF2IP/RAP1; the interaction is direct. Ubiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; DNA repair, damage

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: nucleoplasm; male germ cell nucleus; Golgi apparatus; chromosome, telomeric region; nuclear telomere cap complex; Mre11 complex; nuclear chromosome, telomeric region; cytoplasm; nucleus

Molecular Function: protein C-terminus binding; protein binding; protein homodimerization activity; telomeric DNA binding; double-stranded telomeric DNA binding; chromatin binding

Biological Process: negative regulation of telomere maintenance; telomere capping; negative regulation of telomere maintenance via semi-conservative replication; in utero embryonic development; telomeric loop formation; positive regulation of nitric-oxide synthase activity; positive regulation of telomere maintenance; telomere maintenance via telomerase; cell cycle; age-dependent telomere shortening; telomere maintenance; protection from non-homologous end joining at telomere