Western Blot (WB)
(Western Blot: Lane1: Rat Brain Tissue; Lane2: Rat Lung Tissue; Lane3: Rat Liver Tissue.)
Rabbit anti-Rat Telomerase Reverse Transcriptase (TERT) Polyclonal Antibody | anti-TERT antibody
Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT)
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Telomerase Reverse Transcriptase (TERT); Polyclonal Antibody; Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT); anti-TERT antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against TERT. It has been selected for its ability to recognize TERT in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-TSDSYRIW RTFVLRVRAL DQTPRMYFVK ADVTGAYDAI PQDKLVEIVA NIIRRSESMY CIRQYAVVQK DSQGQVHKSF RRQVSTLSDL QPYMGQFTKH LQDSDASALR NSVVIEQSIS MNETGSSLLH FFLRFVRHSV VKIDGRFYVQ CQGIPQGSSL STLLCSLCFG DMENKLFAEV QQDGLLLRFV DDFLLVTPHL AHAKAFLSTL VHGVPEYGCM INLQKTVVNF PVETGALGGA APHQLPAHCL FPWCGLLLDT RTLEVFCDYS GYGRTSIKMS LTFQGVSRAG KTMRYKLLSV LRLKCH
MGHHHHHHSG SEF-TSDSYRIW RTFVLRVRAL DQTPRMYFVK ADVTGAYDAI PQDKLVEIVA NIIRRSESMY CIRQYAVVQK DSQGQVHKSF RRQVSTLSDL QPYMGQFTKH LQDSDASALR NSVVIEQSIS MNETGSSLLH FFLRFVRHSV VKIDGRFYVQ CQGIPQGSSL STLLCSLCFG DMENKLFAEV QQDGLLLRFV DDFLLVTPHL AHAKAFLSTL VHGVPEYGCM INLQKTVVNF PVETGALGGA APHQLPAHCL FPWCGLLLDT RTLEVFCDYS GYGRTSIKMS LTFQGVSRAG KTMRYKLLSV LRLKCH
Sequence Length
1191
Applicable Applications for anti-TERT antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant TERT (Thr673~His976) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2064210
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Lane1: Rat Brain Tissue; Lane2: Rat Lung Tissue; Lane3: Rat Liver Tissue.)
Western Blot (WB)
(Western Blot: Lane1: Rat Brain Tissue; Lane2: Rat Lung Tissue; Lane3: Rat Liver Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant TERT, Rat.)
Western Blot (WB)
(Western Blot: Sample: Recombinant TERT, Rat.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68,614 Da
NCBI Official Full Name
telomerase reverse transcriptase
NCBI Official Synonym Full Names
telomerase reverse transcriptase
NCBI Official Symbol
Tert
NCBI Protein Information
telomerase reverse transcriptase
UniProt Protein Name
Telomerase reverse transcriptase
Protein Family
UniProt Gene Name
Tert
Uniprot Description
Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis ().
Research Articles on TERT
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Product Notes
The TERT tert (Catalog #AAA2005090) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Telomerase Reverse Transcriptase (TERT) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the TERT tert for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-TSDSYR IW RTFVLRVRAL DQTPRMYFVK ADVTGAYDAI PQDKLVEIVA NIIRRSESMY CIRQYAVVQK DSQGQVHKSF RRQVSTLSDL QPYMGQFTKH LQDSDASALR NSVVIEQSIS MNETGSSLLH FFLRFVRHSV VKIDGRFYVQ CQGIPQGSSL STLLCSLCFG DMENKLFAEV QQDGLLLRFV DDFLLVTPHL AHAKAFLSTL VHGVPEYGCM INLQKTVVNF PVETGALGGA APHQLPAHCL FPWCGLLLDT RTLEVFCDYS GYGRTSIKMS LTFQGVSRAG KTMRYKLLSV LRLKCH. It is sometimes possible for the material contained within the vial of "Telomerase Reverse Transcriptase (TERT), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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