Rat Interleukin 17F (IL17F) ELISA Kit | IL17F elisa kit

Rat Interleukin 17F (IL17F) ELISA Kit

Cat. Num. AAA2703953

• Gene Names: IL17F; ML1; ML-1; CANDF6; IL-17F
• Reactivity: Rat
• Synonyms: Interleukin 17F (IL17F); Rat Interleukin 17F (IL17F) ELISA Kit; ML-1; IL17F elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 17F (IL17F).; No significant cross-reactivity or interference between Interleukin 17F (IL17F) and analogues was observed.
• Sequence Length: 163

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 5.9pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 17F (IL17F) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 17F (IL17F) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL17F elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 17F (IL17F). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 17F (IL17F). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 17F (IL17F), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 17F (IL17F) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 16418375
• NCBI GeneID: 112744
• NCBI Accession #: NP_443104.1
• NCBI GenBank Nucleotide #: NM_052872.3
• UniProt Accession #: Q96PD4; Q6NSI0; Q7Z6P4; Q96PI8; Q9NUE6
• Molecular Weight: 18,045 Da
• NCBI Official Full Name: interleukin-17F
• NCBI Official Synonym Full Names: interleukin 17F
• NCBI Official Symbol: IL17F
• NCBI Official Synonym Symbols: ML1; ML-1; CANDF6; IL-17F
• NCBI Protein Information: interleukin-17F
• UniProt Protein Name: Interleukin-17F
• Protein Family: Interleukin
• UniProt Gene Name: IL17F
• UniProt Synonym Gene Names: IL-17F

NCBI Description

The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. [provided by RefSeq, Jul 2008]

Uniprot Description

Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover (PubMed:11591732, PubMed:11591768, PubMed:11574464). Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis (PubMed:11591732). Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation ().