GGCT recombinant protein

GGCT Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121676

• Gene Names: GGCT; GGC; GCTG; CRF21; C7orf24
• Purity: >95% as determined by SDS-PAGE
• Synonyms: GGCT; GGCT Protein; Human; Recombinant (His Tag); Human GGCT Protein (His Tag); C7orf24 Protein; CRF21 Protein; GCTG Protein; GGC Protein; gamma-glutamylcyclotransferase; GGCT recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu188

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mature form of human GGCT (O75223-1)(Met1-Leu188) was expressed with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GGCT recombinant protein

Background: GGCT belongs to the gamma-glutamylcyclotransferase family. It catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides, the penultimate step in glutathione catabolism. GGCT may play a significant role in glutathione homeostasis. GGCT also induces release of cytochrome c from mitochondria with resultant induction of apoptosis. Pseudogenes of GGCT gene are located on the long arm of chromosome 5 and the short arm of chromosomes 2 and 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

References

Wagner SA. et al., 2011, Mol Cell Proteomics. 10 (10): M111.013284. Kim W. et al., 2011, Mol Cell. 44 (2): 325-40. Amano T. et al., 2012, J Histochem Cytochem. 60 (1): 76-86.

NCBI and Uniprot Product Information

• NCBI GI #: 315360618
• NCBI GeneID: 79017
• NCBI Accession #: NP_001186744.1
• NCBI GenBank Nucleotide #: NM_001199815.1
• UniProt Accession #: O75223; Q9BS37; B2RDN0; B8ZZN4; B8ZZR8
• Molecular Weight: 19,092 Da
• NCBI Official Full Name: gamma-glutamylcyclotransferase isoform 2
• NCBI Official Synonym Full Names: gamma-glutamylcyclotransferase
• NCBI Official Symbol: GGCT
• NCBI Official Synonym Symbols: GGC; GCTG; CRF21; C7orf24
• NCBI Protein Information: gamma-glutamylcyclotransferase; cytochrome c-releasing factor 21; gamma -glutamyl cyclotransferase
• UniProt Protein Name: Gamma-glutamylcyclotransferase
• Protein Family: Gamma-glutamylcyclotransferase
• UniProt Gene Name: GGCT
• UniProt Synonym Gene Names: C7orf24; CRF21
• UniProt Entry Name: GGCT_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides, the penultimate step in glutathione catabolism, and may play a critical role in glutathione homeostasis. The encoded protein may also play a role in cell proliferation, and the expression of this gene is a potential marker for cancer. Pseudogenes of this gene are located on the long arm of chromosome 5 and the short arm of chromosomes 2 and 20. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

GGCT: Catalyzes the formation of 5-oxoproline from gamma- glutamyl dipeptides and may play a significant role in glutathione homeostasis. Induces release of cytochrome c from mitochondria with resultant induction of apoptosis. Belongs to the gamma-glutamylcyclotransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; Other Amino Acids Metabolism - glutathione; EC 2.3.2.4; Apoptosis

Chromosomal Location of Human Ortholog: 7p15-p14

Cellular Component: cytosol

Molecular Function: gamma-glutamylcyclotransferase activity; protein homodimerization activity

Biological Process: release of cytochrome c from mitochondria; xenobiotic metabolic process; glutathione biosynthetic process