Rabbit anti-Human, Mouse TEAD2 Polyclonal Antibody | anti-TEAD2 antibody

TEAD2, ID (TEAD2, TEF4, Transcriptional enhancer factor TEF-4, TEA domain family member 2) (AP)

Cat. Num. AAA6354678

• Gene Names: TEAD2; ETF; TEF4; TEF-4; TEAD-2
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TEAD2; Polyclonal Antibody; ID (TEAD2; TEF4; Transcriptional enhancer factor TEF-4; TEA domain family member 2) (AP); TEA domain family member 2; anti-TEAD2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-TEAD2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TEAD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-295 amino acids from the Central region of human TEAD2.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TEAD2 Antibody (Center) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the TEAD2 antibody detected the TEAD2 protein (arrow).)

Western Blot (WB)

(TEAD2 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the TEAD2 antibody detected the TEAD2 protein (arrow).)

Related Product Information for anti-TEAD2 antibody

Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.

Product Categories/Family for anti-TEAD2 antibody

Antibodies; Transcription Factors

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Tian, W., et al. Proc. Natl. Acad. Sci. U.S.A. 107(16):7293-7298(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Zhang, H., et al. J. Biol. Chem. 284(20):13355-13362(2009). Zhao, B., et al. Genes Dev. 22(14):1962-1971(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 20070102
• NCBI GeneID: 8463
• NCBI Accession #: NP_003589.1
• NCBI GenBank Nucleotide #: NM_003598
• UniProt Accession #: Q15562; Q8NA25; Q96IG3; B4DTJ6; M0R1T9
• Molecular Weight: 49,640 Da
• NCBI Official Full Name: Homo sapiens TEA domain family member 2 (TEAD2), transcript variant 5, mRNA
• NCBI Official Synonym Full Names: TEA domain family member 2
• NCBI Official Symbol: TEAD2
• NCBI Official Synonym Symbols: ETF; TEF4; TEF-4; TEAD-2
• NCBI Protein Information: transcriptional enhancer factor TEF-4
• UniProt Protein Name: Transcriptional enhancer factor TEF-4
• Protein Family: Transcriptional enhancer factor
• UniProt Gene Name: TEAD2
• UniProt Synonym Gene Names: TEF4; TEAD-2
• UniProt Entry Name: TEAD2_HUMAN

Uniprot Description

TEAD2: Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'- GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif. Interacts with YAP1 and WWTR1/TAZ.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; transcription factor complex; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; DNA binding; transcription factor activity

Biological Process: paraxial mesoderm development; transcription initiation from RNA polymerase II promoter; regulation of transcription, DNA-dependent; neural tube closure; lateral mesoderm development; gene expression; positive regulation of transcription from RNA polymerase II promoter; vasculogenesis; notochord development