Western Blot (WB)
(TDG / Thymine-DNA glycosylase antibody (pAb) tested by Western blot Nuclear extract of P19 cells (30 ug) probed with TDG / Thymine-DNA glycosylase antibody (1:500).)
Rabbit anti-Mouse TDG/Thymine-DNA glycosylase Polyclonal Antibody | anti-TDG antibody
TDG/Thymine-DNA glycosylase antibody (pAb)
Reactivity
Mouse
Applications
Western Blot
Purity
Protein A Chromatography
Synonyms
TDG/Thymine-DNA glycosylase; Polyclonal Antibody; TDG/Thymine-DNA glycosylase antibody (pAb); Jza1; JZA-3; E130317C12Rik; anti-TDG antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-TDG antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:500-1:1,500 dilution
WB: 1:500-1:1,500 dilution
Immunogen
This antibody was raised against a full-length recombinant protein corresponding to mouse thymine DNA glycosylase (TDG).
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(TDG / Thymine-DNA glycosylase antibody (pAb) tested by Western blot Nuclear extract of P19 cells (30 ug) probed with TDG / Thymine-DNA glycosylase antibody (1:500).)
Western Blot (WB)
(TDG / Thymine-DNA glycosylase antibody (pAb) tested by Western blot Nuclear extract of P19 cells (30 ug) probed with TDG / Thymine-DNA glycosylase antibody (1:500).)
Related Product Information for anti-TDG antibody
Short Description: Our TDG/Thymine-DNA glycosylase antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Mouse samples.
Background: In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. In addition, TET proteins oxidize 5- methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5- formylcytosine (5fC), and 5-carboxylcytosine (5caC). TDG/Thymine-DNA glycosylase functions to correct G/T mispairs to G/C pairs and plays a role in active DNA demethylation by recognizing 5fC and 5caC and converting these to cystosine through the base excision repair (BER) pathway.
Background: In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. In addition, TET proteins oxidize 5- methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5- formylcytosine (5fC), and 5-carboxylcytosine (5caC). TDG/Thymine-DNA glycosylase functions to correct G/T mispairs to G/C pairs and plays a role in active DNA demethylation by recognizing 5fC and 5caC and converting these to cystosine through the base excision repair (BER) pathway.
Product Categories/Family for anti-TDG antibody
NCBI and Uniprot Product Information
Molecular Weight
55kDa
Protein Family
Similar Products
Product Notes
The TDG (Catalog #AAA388896) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TDG/Thymine-DNA glycosylase antibody (pAb) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TDG/Thymine-DNA glycosylase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:500-1:1,500 dilution. Researchers should empirically determine the suitability of the TDG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TDG/Thymine-DNA glycosylase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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