Rabbit anti-Human, Mouse G/T mismatch-specific thymine DNA glycosylase Polyclonal Antibody | anti-TDG antibody

Rabbit anti-human G/T mismatch-specific thymine DNA glycosylase polyclonal Antibody(TDG)

Cat. Num. AAA1498143

• Gene Names: TDG; hTDG
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: G/T mismatch-specific thymine DNA glycosylase; Polyclonal Antibody; Rabbit anti-human G/T mismatch-specific thymine DNA glycosylase polyclonal Antibody(TDG); Thymine-DNA glycosylase; hTDG; TDG; anti-TDG antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 410

• Applicable Applications for anti-TDG antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: WB:1:500-2000; IHC:1:20-1:200
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human G/T mismatch-specific thymine DNA glycosylase protein(141-410aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-22845 / sc-22847 / sc-292440
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(All lanes: G/T mismatch-specific thymine DNA glycosylase antibody at 6ug/ml+mouse brain tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 46kDaObserved band size: 46kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human heart using MBS1498143 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon cancer using MBS1498143 at dilution 1:100)

Related Product Information for anti-TDG antibody

DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine. Cannot remove 5-hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.

NCBI and Uniprot Product Information

• NCBI GI #: 59853162
• NCBI GeneID: 6996
• NCBI Accession #: NP_003202.3
• NCBI GenBank Nucleotide #: NM_003211.4
• UniProt Accession #: Q13569; Q8IUZ6; Q8IZM3
• Molecular Weight: 46,053 Da
• NCBI Official Full Name: G/T mismatch-specific thymine DNA glycosylase
• NCBI Official Synonym Full Names: thymine DNA glycosylase
• NCBI Official Symbol: TDG
• NCBI Official Synonym Symbols: hTDG
• NCBI Protein Information: G/T mismatch-specific thymine DNA glycosylase
• UniProt Protein Name: G/T mismatch-specific thymine DNA glycosylase
• Protein Family: G/T mismatch-specific thymine DNA glycosylase
• UniProt Gene Name: TDG
• UniProt Synonym Gene Names: hTDG
• UniProt Entry Name: TDG_HUMAN

NCBI Description

The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq, Jul 2008]

Uniprot Description

TDG: In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. This enzyme corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar- phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. Belongs to the TDG/mug DNA glycosylase family.

Protein type: EC 3.2.2.29; Hydrolase; DNA repair, damage; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 12q24.1

Cellular Component: nucleoplasm; nucleus; plasma membrane; PML body

Molecular Function: damaged DNA binding; DNA N-glycosylase activity; double-stranded DNA binding; mismatched DNA binding; protein binding; protein homodimerization activity; protein kinase C binding; pyrimidine-specific mismatch base pair DNA N-glycosylase activity; structure-specific DNA binding; SUMO binding; uracil DNA N-glycosylase activity

Biological Process: base-excision repair; base-excision repair, AP site formation; cellular protein metabolic process; chromatin modification; depyrimidination; DNA repair; embryonic development; gene expression; mismatch repair; negative regulation of protein binding; negative regulation of transcription from RNA polymerase II promoter; post-translational protein modification; protein sumoylation; regulation of gene expression, epigenetic; transcription, DNA-dependent