Rabbit anti-Human TBPL2 Polyclonal Antibody | anti-TBPL2 antibody

TBPL2, NT (TBPL2, TBP2, TRF3, TATA box-binding protein-like protein 2, TATA box-binding protein-related factor 3) (Biotin)

Cat. Num. AAA6354130

• Gene Names: TBPL2; TBP2; TRF3
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TBPL2; Polyclonal Antibody; NT (TBPL2; TBP2; TRF3; TATA box-binding protein-like protein 2; TATA box-binding protein-related factor 3) (Biotin); TATA box-binding protein-related factor 3; anti-TBPL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TBPL2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: TBPL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-47 amino acids from the N-terminal region of human TBPL2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TBPL2 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TBPL2 antibody detected the TBPL2 protein (arrow).)

Immunohistochemistry (IHC)

(TBPL2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TBPL2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Fluorescent confocal image of Hela cell stained with TBPL2 Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBPL2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TBPL2 immunoreactivity is localized to Nucleus and Cytoplasm significantly.)

Related Product Information for anti-TBPL2 antibody

TBPL2 is a transcription factor required in complex with TAF3 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process (By similarity).

Product Categories/Family for anti-TBPL2 antibody

Antibodies; Binding Proteins

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Deato, M.D., et al. Genes Dev. 21(17):2137-2149(2007). Di Pietro, C., et al. DNA Cell Biol. 26(6):369-385(2007). Persengiev, S.P., et al. Proc. Natl. Acad. Sci. U.S.A. 100(25):14887-14891(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 39930602
• NCBI GeneID: 387332
• NCBI Accession #: NP_950248.1
• NCBI GenBank Nucleotide #: NM_199047.2
• UniProt Accession #: Q6SJ96; Q17RU8
• Molecular Weight: 41,524 Da
• NCBI Official Full Name: TATA box-binding protein-like protein 2
• NCBI Official Synonym Full Names: TATA box binding protein like 2
• NCBI Official Symbol: TBPL2
• NCBI Official Synonym Symbols: TBP2; TRF3
• NCBI Protein Information: TATA box-binding protein-like protein 2; TBP-like protein 2; TBP-related factor 3; TATA box-binding protein-related factor 3
• UniProt Protein Name: TATA box-binding protein-like protein 2
• Protein Family: TATA box-binding protein-like protein
• UniProt Gene Name: TBPL2
• UniProt Synonym Gene Names: TBP2; TRF3; TBP-like protein 2
• UniProt Entry Name: TBPL2_HUMAN

Uniprot Description

TBPL2: Transcription factor required in complex with TAF3 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process. Belongs to the TBP family.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 14q22.3

Cellular Component: cytoplasm; nucleus

Molecular Function: DNA binding; transcription factor activity

Biological Process: multicellular organismal development; transcription initiation; negative regulation of transcription, DNA-dependent