Western Blot (WB)
(BIN2 Antibody (N-term) western blot analysis in HL-60,CEM,MDA-MB435,A549 cell line lysates (15ug/lane).This demonstrates the BIN2 antibody detected the BIN2 protein (arrow).)
Rabbit anti-Human BIN2 Polyclonal Antibody | anti-BIN2 antibody
BIN2 Antibody (N-term)
Gene Names
BIN2; BRAP-1
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
BIN2; Polyclonal Antibody; BIN2 Antibody (N-term); Bridging integrator 2; Breast cancer-associated protein 1; BRAP1; anti-BIN2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This BIN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-42 amino acids from the N-terminal region of human BIN2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
14-42
Sequence Length
565
Applicable Applications for anti-BIN2 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(BIN2 Antibody (N-term) western blot analysis in HL-60,CEM,MDA-MB435,A549 cell line lysates (15ug/lane).This demonstrates the BIN2 antibody detected the BIN2 protein (arrow).)
Western Blot (WB)
(BIN2 Antibody (N-term) western blot analysis in HL-60,CEM,MDA-MB435,A549 cell line lysates (15ug/lane).This demonstrates the BIN2 antibody detected the BIN2 protein (arrow).)
Immunohistochemistry (IHC)
(BIN2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BIN2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(BIN2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BIN2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(BIN2 Antibody (N-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(BIN2 Antibody (N-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-BIN2 antibody
References
Scherer, S.E., et al. Nature 440(7082):346-351(2006)
Ge, K., et al. Genomics 67(2):210-220(2000)
Ge, K., et al. Genomics 67(2):210-220(2000)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61874
NCBI Official Full Name
bridging integrator 2
NCBI Official Synonym Full Names
bridging integrator 2
NCBI Official Symbol
BIN2
NCBI Official Synonym Symbols
BRAP-1
NCBI Protein Information
bridging integrator 2
UniProt Protein Name
Bridging integrator 2
Protein Family
UniProt Gene Name
BIN2
UniProt Synonym Gene Names
BRAP1
UniProt Entry Name
BIN2_HUMAN
Uniprot Description
BIN2: 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 12q13
Cellular Component: cell projection; podosome; cell cortex; cell junction; phagocytic cup
Molecular Function: protein binding; phospholipid binding
Biological Process: phagocytosis, engulfment
Research Articles on BIN2
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Product Notes
The BIN2 bin2 (Catalog #AAA9206689) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 14-42. The BIN2 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BIN2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the BIN2 bin2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BIN2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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