Rabbit anti-Human TAF8 Polyclonal Antibody | anti-TAF8 antibody

TAF8, ID (TAF8, TAFII43, TBN, Transcription initiation factor TFIID subunit 8, Protein taube nuss, TBP-associated factor 43kD, TBP-associated factor 8, Transcription initiation factor TFIID 43kD subunit) (Biotin)

Cat. Num. AAA6353437

• Gene Names: TAF8; 43; II; TAF; TBN; TAFII43; TAFII-43
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TAF8; Polyclonal Antibody; ID (TAF8; TAFII43; TBN; Transcription initiation factor TFIID subunit 8; Protein taube nuss; TBP-associated factor 43kD; TBP-associated factor 8; Transcription initiation factor TFIID 43kD subunit) (Biotin); Transcription initiation factor TFIID 43kD subunit; anti-TAF8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TAF8 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: TAF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-178 amino acids from the Central region of human TAF8.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TAF8 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TAF8 antibody detected the TAF8 protein (arrow).)

Immunohistochemistry (IHC)

(TAF8 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TAF8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-TAF8 antibody

This gene encodes one of several TATA-binding protein (TBP)-associated factors (TAFs), which are integral subunits of the general transcription factor complex TFIID. TFIID recognizes the core promoter of many genes and nucleates the assembly of a transcription preinitiation complex containing RNA polymerase II and other initiation factors. The protein encoded by this gene contains an H4-like histone fold domain, and interacts with several subunits of TFIID including TBP and the histone-fold protein TAF10. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq].

Product Categories/Family for anti-TAF8 antibody

Antibodies; Transcription Factors

References

Ganesh, S.K., et al. Nat. Genet. 41(11):1191-1198(2009). Soranzo, N., et al. Nat. Genet. 41(11):1182-1190(2009). Chapuis, J., et al. Mol. Psychiatry 14(11):1004-1016(2009). Soutoglou, E., et al. Mol. Cell. Biol. 25(10):4092-4104(2005). Guermah, M., et al. Mol. Cell 12(4):991-1001(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 104485446
• NCBI GeneID: 129685
• NCBI Accession #: NP_612639.2
• NCBI GenBank Nucleotide #: NM_138572.2
• UniProt Accession #: Q7Z7C8; Q5T0K1; Q8N4R9; Q96M52
• Molecular Weight: 37,411 Da
• NCBI Official Full Name: transcription initiation factor TFIID subunit 8
• NCBI Official Synonym Full Names: TAF8 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 43kDa
• NCBI Official Symbol: TAF8
• NCBI Official Synonym Symbols: 43; II; TAF; TBN; TAFII43; TAFII-43
• NCBI Protein Information: transcription initiation factor TFIID subunit 8; hTAFII43; protein taube nuss; taube nuss homolog; TBP-associated factor 8; TBP-associated factor 43 kDa; TBP-associated factor TAFII43; TBP-associated factor, RNA polymerase II, 43 kD; transcription initiat
• UniProt Protein Name: Transcription initiation factor TFIID subunit 8
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF8
• UniProt Synonym Gene Names: TAFII43; TBN; TAFII-43; TAFII43; hTAFII43

NCBI Description

This gene encodes one of several TATA-binding protein (TBP)-associated factors (TAFs), which are integral subunits of the general transcription factor complex TFIID. TFIID recognizes the core promoter of many genes and nucleates the assembly of a transcription preinitiation complex containing RNA polymerase II and other initiation factors. The protein encoded by this gene contains an H4-like histone fold domain, and interacts with several subunits of TFIID including TBP and the histone-fold protein TAF10. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TBN: Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however, does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex. May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo. Belongs to the TAF8 family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: nucleoplasm; nucleus; perinuclear region of cytoplasm; transcription factor TFIID complex

Molecular Function: protein binding

Biological Process: maintenance of protein localization in nucleus; positive regulation of transcription, DNA-dependent; regulation of fat cell differentiation; snRNA transcription from RNA polymerase II promoter