Western Blot (WB)
(Western blot analysis of SamD12 expression in human HEK293T transfected cells. (1) empty vector; (2) SamD10; (3) SamD12. (Anti-SamD12 was used at a 1:1000 dilution.))
Rabbit anti-Human, Mouse SamD12 Polyclonal Antibody | anti-SamD12 antibody
SamD12 Rabbit Polyclonal Antibody
Reactivity
Human, Mouse
Applications
Western Blot
Synonyms
SamD12; Polyclonal Antibody; SamD12 Rabbit Polyclonal Antibody; Sterile alpha motif (SAM) domain-containing protein 12; Hyphen D12; HYPD12; Aveugle; anti-SamD12 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody recognizes the protein SamD12. The antibody does not cross-react with SamD10.
Form/Format
Lyophilized serum
Sequence Length
201
Applicable Applications for anti-SamD12 antibody
Western Blot (WB)
Application Notes
If reconstituted in 100 uL: WB: 1:1000.
Optimal dilution has to determined by the end user.
Optimal dilution has to determined by the end user.
Preservative
None
Preparation and Storage
Lyophilized antibody can be kept at 4 degree C for up to 3 months and should be kept at-20 degree C for long-term storage.
To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C).
For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C).
For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of SamD12 expression in human HEK293T transfected cells. (1) empty vector; (2) SamD10; (3) SamD12. (Anti-SamD12 was used at a 1:1000 dilution.))
Western Blot (WB)
(Western blot analysis of SamD12 expression in human HEK293T transfected cells. (1) empty vector; (2) SamD10; (3) SamD12. (Anti-SamD12 was used at a 1:1000 dilution.))
Related Product Information for anti-SamD12 antibody
SamD10 and SamD12 are two recently discovered homolog proteins consisting of a SAM domain. These proteins are also known as Hyphen (HYP) or Aveugle (Ave) and they have been demonstrated to interact with the SAM domain of the Connector enhancer of KSR (CNK) downstream of RAS but a step upstream of RAF. A role for these proteins downstream of EGFR during eye and wing development of Drosophila has also been demonstrated. Heterodimerization of CNK/SAM has been shown to facilitate the recruitment of a KSR/MEK complex to CNK and bridge KSR/MEK to RAF which greatly enhances RAS-mediated RAF activation.
References
1. Claperon A and Therrien M-KSR and CNK: two scaffolds regulating RAS-mediated RAF activation. 2. Douziech M-A KSR/CNK complex mediated by HYP, a novel SAM domain-containing protein, regulates RAS-dependent RAF activation in Drosophila. 3. Roignant JY-The novel SAM domain protein Aveugle is required for Raf activation in the Drosophila EGF receptor signaling pathway.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
SAMD12, partial
Protein Family
Similar Products
Product Notes
The SamD12 (Catalog #AAA588224) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SamD12 Rabbit Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SamD12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). If reconstituted in 100 uL: WB: 1:1000. Optimal dilution has to determined by the end user. Researchers should empirically determine the suitability of the SamD12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SamD12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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