Western Blot (WB)
(Western blot analysis of lysates from Hela, U-251 MG cell line (from left to right), using TAF8 Antibody (Center). MBS9213534 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Rabbit TAF8 Polyclonal Antibody | anti-TAF8 antibody
TAF8 Antibody (Center)
Western Blot (WB)
(Western blot analysis of lysates from Hela, U-251 MG cell line (from left to right), using TAF8 Antibody (Center). MBS9213534 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from Hela, U-251 MG cell line (from left to right), using TAF8 Antibody (Center). MBS9213534 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Immunohistochemistry (IHC)
(TAF8 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TAF8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(TAF8 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TAF8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
(TBP)-associated factors (TAFs), which are integral subunits of the
general transcription factor complex TFIID. TFIID recognizes the
core promoter of many genes and nucleates the assembly of a
transcription preinitiation complex containing RNA polymerase II
and other initiation factors. The protein encoded by this gene
contains an H4-like histone fold domain, and interacts with several
subunits of TFIID including TBP and the histone-fold protein TAF10.
Alternatively spliced transcript variants have been described, but
their biological validity has not been determined. [provided by
RefSeq].
Soranzo, N., et al. Nat. Genet. 41(11):1182-1190(2009)
Chapuis, J., et al. Mol. Psychiatry 14(11):1004-1016(2009)
Soutoglou, E., et al. Mol. Cell. Biol. 25(10):4092-4104(2005)
Guermah, M., et al. Mol. Cell 12(4):991-1001(2003)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one of several TATA-binding protein (TBP)-associated factors (TAFs), which are integral subunits of the general transcription factor complex TFIID. TFIID recognizes the core promoter of many genes and nucleates the assembly of a transcription preinitiation complex containing RNA polymerase II and other initiation factors. The protein encoded by this gene contains an H4-like histone fold domain, and interacts with several subunits of TFIID including TBP and the histone-fold protein TAF10. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
TBN: Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however, does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex. May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo. Belongs to the TAF8 family. 3 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 6p21.1
Cellular Component: nucleoplasm; transcription factor TFIID complex; perinuclear region of cytoplasm; nucleus
Molecular Function: protein binding; protein heterodimerization activity
Biological Process: transcription, DNA-dependent; regulation of fat cell differentiation; positive regulation of transcription, DNA-dependent; maintenance of protein localization in nucleus; cell differentiation; inner cell mass cell proliferation
Research Articles on TAF8
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Product Notes
The TAF8 taf8 (Catalog #AAA9213534) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 151-178. The TAF8 Antibody (Center) reacts with Human (Predicted Reactivity: Bovine, Chicken) and may cross-react with other species as described in the data sheet. AAA Biotech's TAF8 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the TAF8 taf8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TAF8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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