Rabbit anti-Human, Mouse TAF1D Polyclonal Antibody | anti-TAF1D antibody

TAF1D, NT (TAF1D, JOSD3, TATA box-binding protein-associated factor RNA polymerase I subunit D, RNA polymerase I-specific TBP-associated factor 41kD, TATA box-binding protein-associated factor 1D, Transcription initiation factor SL1/TIF-IB subunit D) (PE)

Cat. Num. AAA6353368

• Gene Names: TAF1D; JOSD3; RAFI41; TAFI41; TAF(I)41
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TAF1D; Polyclonal Antibody; NT (TAF1D; JOSD3; TATA box-binding protein-associated factor RNA polymerase I subunit D; RNA polymerase I-specific TBP-associated factor 41kD; TATA box-binding protein-associated factor 1D; Transcription initiation factor SL1/TIF-IB subunit D) (PE); Transcription initiation factor SL1/TIF-IB subunit D; anti-TAF1D antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-TAF1D antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: TAF1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-98 amino acids from the N-terminal region of human TAF1D.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TAF1D Antibody (N-term) western blot analysis in NCI-H292,Jurkat cell line and mouse liver lysates (15ug/lane).This demonstrates the TAF1D antibody detected the TAF1D protein (arrow).)

Immunohistochemistry (IHC)

(TAF1D Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TAF1D Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(TAF1D Antibody (N-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TAF1D antibody

TAF1D is a member of the SL1 complex, which includes TBP (MIM 600075) and TAF1A (MIM 604903), TAF1B (MIM 604904), and TAF1C (MIM 604905), and plays a role in RNA polymerase I transcription (Wang et al., 2004 [PubMed 15520167]; Gorski et al., 2007 [PubMed 17318177]).

Product Categories/Family for anti-TAF1D antibody

Antibodies; Transcription Factors

References

Gorski, J.J., et al. EMBO J. 26(6):1560-1568(2007). Wang, L., et al. Cancer Res. 64(21):7673-7677(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 13129136
• NCBI GeneID: 79101
• NCBI Accession #: NP_077021.1
• NCBI GenBank Nucleotide #: NM_024116.3
• UniProt Accession #: Q9H5J8; Q6I9Y6
• Molecular Weight: 32,058 Da
• NCBI Official Full Name: TATA box-binding protein-associated factor RNA polymerase I subunit D
• NCBI Official Synonym Full Names: TATA box binding protein (TBP)-associated factor, RNA polymerase I, D, 41kDa
• NCBI Official Symbol: TAF1D
• NCBI Official Synonym Symbols: JOSD3; RAFI41; TAFI41; TAF(I)41
• NCBI Protein Information: TATA box-binding protein-associated factor RNA polymerase I subunit D; TBP-associated factor 1D; Josephin domain containing 3; TATA box-binding protein-associated factor 1D; transcription initiation factor SL1/TIF-IB subunit D; RNA polymerase I-specific T
• UniProt Protein Name: TATA box-binding protein-associated factor RNA polymerase I subunit D
• Protein Family: TATA box-binding protein-associated factor
• UniProt Gene Name: TAF1D
• UniProt Synonym Gene Names: JOSD3; TAFI41; TBP-associated factor 1D
• UniProt Entry Name: TAF1D_HUMAN