Rabbit NTRK1 Polyclonal Antibody | anti-NTRK1 antibody

NTRK1 Polyclonal Antibody

Cat. Num. AAA126348

• Gene Names: NTRK1; MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: NTRK1; Polyclonal Antibody; NTRK1 Polyclonal Antibody; MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA; anti-NTRK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 796

• Applicable Applications for anti-NTRK1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50-1:200
• Category: Primary antibody
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human TrkA (NP_002520.2).
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using TrkA antibody (MBS126348) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.)

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using TrkA antibody (MBS126348) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse stomach using NTRK1 Antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat cerebellum using NTRK1 Antibody at dilution of 1:100 (40x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cells using TRKA antibody (MBS126348) at dilution of 1:100. Blue: DAPI for nuclear staining.)

Related Product Information for anti-NTRK1 antibody

The family of Trk receptor tyrosine kinases consists of TrkA, TrkB, and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3 (1). Neurotrophin signaling through these receptors regulates a number of physiological processes, such as cell survival, proliferation, neural development, and axon and dendrite growth and patterning (1). In the adult nervous system, the Trk receptors regulate synaptic strength and plasticity. TrkA regulates proliferation and is important for development and maturation of the nervous system (2). Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade (3,4). Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at these sites reflects TrkA kinase activity (3-6). Point mutations, deletions, and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA (7-10). TrkA is activated in many malignancies including breast, ovarian, prostate, and thyroid carcinomas (8-13). Research studies suggest that expression of TrkA in neuroblastomas may be a good prognostic marker as TrkA signals growth arrest and differentiation of cells originating from the neural crest (10).

Product Categories/Family for anti-NTRK1 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 94730402
• NCBI GeneID: 4914
• UniProt Accession #: P04629; P08119; Q15655; Q15656; Q5D056; Q5VZS2; Q7Z5C3; Q9UIU7; B2R6T5; B7ZM34
• Molecular Weight: Observed MW: 120kDa; Calculated MW: 77kDa/83kDa/86kDa/87kDa
• NCBI Official Full Name: High affinity nerve growth factor receptor
• NCBI Official Synonym Full Names: neurotrophic tyrosine kinase, receptor, type 1
• NCBI Official Symbol: NTRK1
• NCBI Official Synonym Symbols: MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA
• NCBI Protein Information: high affinity nerve growth factor receptor; gp140trk; Oncogene TRK; tyrosine kinase receptor A; tropomyosin-related kinase A; TRK1-transforming tyrosine kinase protein
• UniProt Protein Name: High affinity nerve growth factor receptor
• Protein Family: High affinity nerve growth factor receptor
• UniProt Gene Name: NTRK1
• UniProt Synonym Gene Names: MTC; TRK; TRKA; Trk-A
• UniProt Entry Name: NTRK1_HUMAN

NCBI Description

This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date. [provided by RefSeq, Jul 2008]

Uniprot Description

TrkA: a receptor tyrosine kinase of the Trk family. High affinity receptor for nerve growth factor, neurotrophin-3 and neurotrophin-4/5 but not brain- derived neurotrophic factor. Known substrates include Shc, PI-3K, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system. Two splice variant isoforms have been described.

Protein type: Protein kinase, TK; Kinase, protein; Oncoprotein; Membrane protein, integral; Protein kinase, tyrosine (receptor); EC 2.7.10.1; TK group; Trk family

Chromosomal Location of Human Ortholog: 1q21-q22

Cellular Component: cell surface; protein complex; integral to plasma membrane; late endosome membrane; early endosome; dendrite; cell soma; axon; early endosome membrane; late endosome; plasma membrane; cytoplasmic vesicle; endosome; receptor complex

Molecular Function: neurotrophin p75 receptor binding; neurotrophin binding; protein binding; nerve growth factor receptor activity; protein homodimerization activity; ephrin receptor binding; nerve growth factor binding; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: circadian rhythm; axon guidance; mechanoreceptor differentiation; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; positive regulation of synaptic transmission, glutamatergic; olfactory nerve development; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; negative regulation of cell proliferation; sympathetic nervous system development; response to radiation; learning and/or memory; small GTPase mediated signal transduction; negative regulation of neuron apoptosis; response to axon injury; response to electrical stimulus; detection of temperature stimulus involved in sensory perception of pain; response to hydrostatic pressure; aging; response to nutrient levels; response to drug; phosphoinositide-mediated signaling; adenylate cyclase activation; Sertoli cell development; detection of mechanical stimulus involved in sensory perception of pain; positive regulation of programmed cell death; positive regulation of angiogenesis; response to ethanol; phospholipase C activation; B cell differentiation; Ras protein signal transduction; positive regulation of Ras protein signal transduction; response to activity; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Thyroid Carcinoma, Familial Medullary; Insensitivity To Pain, Congenital, With Anhidrosis