Rabbit T2R1 Polyclonal Antibody | anti-T2R1 antibody

T2R1 (D45) Polyclonal Antibody

Cat. Num. AAA3003244

• Gene Names: TAS2R1; T2R1; TRB7
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: T2R1; Polyclonal Antibody; T2R1 (D45) Polyclonal Antibody; Taste receptor type 2 member 1; Taste receptor family B member 7; TRB7; TAS2R1; anti-T2R1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: T2R1 (D45) polyclonal antibody detects endogenous levels of T2R1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 299

• Applicable Applications for anti-T2R1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 12-60 of Human T2R1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of T2R1 (D45) pAb at 1:500 dilutionLane1:K562 whole cell lysate (40ug) Lane2:MEF whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug))

Related Product Information for anti-T2R1 antibody

The sense of taste is essential for the survival of organisms. For example, the ability to identify sweet-tasting foods enables animals to seek out food with high nutritive value, whereas the ability to identify bitter substances enables them to avoid the ingestion of potentially harmful substances. A family of integral membrane proteins are involved in taste perception and include T1R, which is involved in sweet taste perception and T2R, which is involved in bitter taste perception. Both types of taste receptors couple to various G proteins to initiate signal transduction cascades. Specifically, T2R1 is expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells.

NCBI and Uniprot Product Information

• NCBI GI #: 9625043
• NCBI GeneID: 50834
• NCBI Accession #: NP_062545.1
• NCBI GenBank Nucleotide #: NP_062545.1
• UniProt Accession #: Q9NYW7
• Molecular Weight: ~ 33kDa
• NCBI Official Full Name: taste receptor type 2 member 1
• NCBI Official Synonym Full Names: taste 2 receptor member 1
• NCBI Official Symbol: TAS2R1
• NCBI Official Synonym Symbols: T2R1; TRB7
• NCBI Protein Information: taste receptor type 2 member 1
• UniProt Protein Name: Taste receptor type 2 member 1
• UniProt Gene Name: TAS2R1
• UniProt Synonym Gene Names: T2R1; TRB7
• UniProt Entry Name: TA2R1_HUMAN

NCBI Description

This gene encodes a member of a family of candidate taste receptors that are members of the G protein-coupled receptor superfamily and that are specifically expressed by taste receptor cells of the tongue and palate epithelia. This intronless taste receptor gene encodes a 7-transmembrane receptor protein, functioning as a bitter taste receptor. This gene is mapped to chromosome 5p15, the location of a genetic locus (PROP) that controls the detection of the bitter compound 6-n-propyl-2-thiouracil. [provided by RefSeq, Jul 2008]

Uniprot Description

TAS2R1: Receptor that may play a role in the perception of bitterness and is gustducin-linked. May play a role in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5. Belongs to the G-protein coupled receptor T2R family.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; GPCR, T2R family; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5p15

Cellular Component: integral to membrane; plasma membrane

Molecular Function: bitter taste receptor activity; taste receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; chemosensory behavior; detection of chemical stimulus involved in sensory perception of bitter taste