Rabbit anti-Human TAS2R1 Polyclonal Antibody | anti-TAS2R1 antibody

TAS2R1, CT (TAS2R1, Taste receptor type 2 member 1, Taste receptor family B member 7) (Azide free) (HRP)

Cat. Num. AAA6353659

• Gene Names: TAS2R1; T2R1; TRB7
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TAS2R1; Polyclonal Antibody; CT (TAS2R1; Taste receptor type 2 member 1; Taste receptor family B member 7) (Azide free) (HRP); Taste receptor family B member 7; anti-TAS2R1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-TAS2R1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TAS2R1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-297 amino acids from the C-terminal region of human TAS2R1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TAS2R1 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the TAS2R1 antibody detected the TAS2R1 protein (arrow).)

Western Blot (WB)

(TAS2R1 Antibody (C-term) western blot analysis in MDA-MB231,293,NCI-H292 cell line lysates (35ug/lane).This demonstrates the TAS2R1 antibody detected the TAS2R1 protein (arrow).)

Related Product Information for anti-TAS2R1 antibody

This gene encodes a member of a family of candidate taste receptors that are members of the G protein-coupled receptor superfamily and that are specifically expressed by taste receptor cells of the tongue and palate epithelia. This intronless taste receptor gene encodes a 7-transmembrane receptor protein, functioning as a bitter taste receptor. This gene is mapped to chromosome 5p15, the location of a genetic locus (PROP) that controls the detection of the bitter compound 6-n-propyl-2-thiouracil.

Product Categories/Family for anti-TAS2R1 antibody

Antibodies; Receptors

References

Upadhyaya, J., et al. Biochem. Biophys. Res. Commun. 398(2):331-335(2010). Weiss, L.A., et al. Nature 461(7265):802-808(2009). Go, Y., et al. Genetics 170(1):313-326(2005). Fischer, A., et al. Mol. Biol. Evol. 22(3):432-436(2005). Zhang, Y., et al. Cell 112(3):293-301(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 9625043
• NCBI GeneID: 50834
• NCBI Accession #: NP_062545.1
• NCBI GenBank Nucleotide #: NM_019599.2
• UniProt Accession #: Q9NYW7; Q646G8
• Molecular Weight: 34,333 Da
• NCBI Official Full Name: taste receptor type 2 member 1
• NCBI Official Synonym Full Names: taste receptor, type 2, member 1
• NCBI Official Symbol: TAS2R1
• NCBI Official Synonym Symbols: T2R1; TRB7
• NCBI Protein Information: taste receptor type 2 member 1; taste receptor family B member 7; taste receptor, family B, member 7
• UniProt Protein Name: Taste receptor type 2 member 1
• Protein Family: Taste receptor
• UniProt Gene Name: TAS2R1
• UniProt Synonym Gene Names: T2R1; TRB7
• UniProt Entry Name: TA2R1_HUMAN

NCBI Description

This gene encodes a member of a family of candidate taste receptors that are members of the G protein-coupled receptor superfamily and that are specifically expressed by taste receptor cells of the tongue and palate epithelia. This intronless taste receptor gene encodes a 7-transmembrane receptor protein, functioning as a bitter taste receptor. This gene is mapped to chromosome 5p15, the location of a genetic locus (PROP) that controls the detection of the bitter compound 6-n-propyl-2-thiouracil. [provided by RefSeq, Jul 2008]

Uniprot Description

TAS2R1: Receptor that may play a role in the perception of bitterness and is gustducin-linked. May play a role in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5. Belongs to the G-protein coupled receptor T2R family.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; GPCR, T2R family; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5p15

Cellular Component: integral to membrane; plasma membrane

Molecular Function: bitter taste receptor activity; taste receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; chemosensory behavior; detection of chemical stimulus involved in sensory perception of bitter taste