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Western Blot (WB) (Western blot analysis of GFPT2 Antibody (Center) in A2058, K562 cell line lysates (35ug/lane). GFPT2 (arrow) was detected using the purified Pab.)

Rabbit GFPT2 Polyclonal Antibody | anti-GFPT2 antibody

GFPT2 Antibody (Center)

Gene Names
GFPT2; GFAT2
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GFPT2; Polyclonal Antibody; GFPT2 Antibody (Center); Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2; D-fructose-6-phosphate amidotransferase 2; Glutamine:fructose-6-phosphate amidotransferase 2; GFAT 2; GFAT2; Hexosephosphate aminotransferase 2; anti-GFPT2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GFPT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-201 amino acids from the Central region of human GFPT2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
175-201
Sequence Length
682
Applicable Applications for anti-GFPT2 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of GFPT2 Antibody (Center) in A2058, K562 cell line lysates (35ug/lane). GFPT2 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of GFPT2 Antibody (Center) in A2058, K562 cell line lysates (35ug/lane). GFPT2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(GFPT2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human skin followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GFPT2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (GFPT2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human skin followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GFPT2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(GFPT2 Antibody (Center) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (GFPT2 Antibody (Center) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-GFPT2 antibody
GFPT2 controls the flux of glucose into the hexosamine pathway. This protein most likely involved in regulating the availability of precursors for N-and O-linked glycosylation of proteins.
Product Categories/Family for anti-GFPT2 antibody
References
Edwards,T.L., et.al., Ann. Hum. Genet. 74 (2), 97-109 (2010)
Prasad,P., et.al., BMC Med. Genet. 11, 52 (2010)
Srinivasan,V., et.al., Clin. Biochem. 40 (13-14), 952-957 (2007)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
76931
NCBI Official Full Name
glutamine--fructose-6-phosphate aminotransferase
NCBI Official Synonym Full Names
glutamine-fructose-6-phosphate transaminase 2
NCBI Official Symbol
GFPT2
NCBI Official Synonym Symbols
GFAT2
NCBI Protein Information
glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2
UniProt Protein Name
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2
UniProt Gene Name
GFPT2
UniProt Synonym Gene Names
GFAT 2; GFAT2
UniProt Entry Name
GFPT2_HUMAN

Uniprot Description

GFAT2: Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Highest levels of expression in heart, placenta, and spinal cord.

Protein type: Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 2.6.1.16; Amino Acid Metabolism - alanine, aspartate and glutamate; Transferase

Chromosomal Location of Human Ortholog: 5q34-q35

Cellular Component: cytosol

Molecular Function: carbohydrate binding; glutamine-fructose-6-phosphate transaminase (isomerizing) activity

Biological Process: cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; energy reserve metabolic process; UDP-N-acetylglucosamine biosynthetic process; glutamine metabolic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; fructose 6-phosphate metabolic process; carbohydrate biosynthetic process

Research Articles on GFPT2

Similar Products

Product Notes

The GFPT2 gfpt2 (Catalog #AAA9213754) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 175-201. The GFPT2 Antibody (Center) reacts with Human (Predicted Reactivity: Bovine, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's GFPT2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the GFPT2 gfpt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFPT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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