Rabbit anti-Rat, Xenopus Synaptotagmin I, p65 Polyclonal Antibody | anti-SYT1 antibody

Synaptotagmin I, p65 (Syt1)

Cat. Num. AAA624740

• Gene Names: SYT1; P65; SYT; SVP65; DKFZp781D2042
• Reactivity: Rat, Xenopus
• Applications: Western Blot, Immunoprecipitation, Immunocytochemistry
• Purity: Serum
• Synonyms: Synaptotagmin I; p65; Polyclonal Antibody; p65 (Syt1); Anti -Synaptotagmin I; anti-SYT1 antibody

• Host: Rabbit
• Reactivity: Rat, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects a 65kD protein, corresponding to the apparent molecular mass of synaptotagmin I on SDS-PAGE immunoblots, in samples from rat and Xenopus. The antibody specificity is confirmed by peptide inhbition as determined by immunoblot analysis.
• Purity/Purification: Serum
• Form/Format: Serum

• Applicable Applications for anti-SYT1 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
• Immunogen: A 23 residue synthetic peptide MVSASHPEALAAPVTTVATLVPH(C) based on the rat synaptotagmin I (residues 1-23) with the cysteine (C) residue added and the peptide coupled to KLH.
• Positive Control: Rat Brain Tissue Extract
• Preparation and Storage: -20 degree C

Related Product Information for anti-SYT1 antibody

Synaptotagmin (also known as p65) is a synaptic vesicle protein that consists of an amino-terminal intravesicular domain, a single transmembrane domain, and a cytoplasmic domain composed of two repeats homologous to the C2 regulatory domain of protein kinase C. Synaptotagmin forms a homo-oligomer that binds calcium in a complex with negatively charged phospholipids. This observation, along with the fact that no synaptotagmin homolog has yet been identified in the constitutive secretory pathway, suggests that synaptotagmin may function as a calcium sensor that triggers exocytosis. The apparent low affinity for calcium and the multiple binding sites for calcium displayed by synaptotagmin are consistent with the properties expected of the calcium trigger for vesicle fusion. Support for a role of synaptotagmin in synaptic vesicle docking of fusion includes direct interactions with syntaxin and neurexin, and either direct or indirect interactions with N-type calcium channels. The interactions with these three presynaptic plasma membrane proteins could potentially position synaptotagmin, and the vesicle, in an optimal position to respond to calcium. Functional data supportive of a role for synaptotagmin in regulated secretion include the inhibition of secretion generated by microinjection of either synaptotagmin peptides into the squid giant synapse, or soluble synaptotagmin fragments and anti-synaptotagmin antibodies into PC-12 cells. However, genetic studies on PC-12 cells, as well as Drosophila and C. elegans, have demonstrated that synaptotagmin is not absolutely required for regulated neurotransmitter release. This may reflect the ability of another protein, such as rabphillin, to compensate partially for the loss of synaptotagmin. Alternatively, synaptotagmin may not be an essential component of the machinery required for vesicle docking and fusion, but rather may function as a calcium-sensitive negative regulator of the constitutive fusion machinery. This possibility is supported by recent electrophysiological recordings from synaptotagmin-deficient Drosophila, which revealed an increase in spontaneous neurotransmitter release and a decrease in evoked release.

Product Categories/Family for anti-SYT1 antibody

Antibodies; Abs to Syntaxin; Synaptotagmin; Synaptophysin

NCBI and Uniprot Product Information

• NCBI GI #: 209447073
• NCBI GeneID: 6857
• NCBI Accession #: NP_001129278.1
• NCBI GenBank Nucleotide #: NM_001135806.1
• UniProt Accession #: P21579; Q6AI31
• Molecular Weight: 47,573 Da
• NCBI Official Full Name: synaptotagmin-1
• NCBI Official Synonym Full Names: synaptotagmin I
• NCBI Official Symbol: SYT1
• NCBI Official Synonym Symbols: P65; SYT; SVP65; DKFZp781D2042
• NCBI Protein Information: synaptotagmin-1; sytI; synaptotagmin 1; OTTHUMP00000166063; OTTHUMP00000166064
• UniProt Protein Name: Synaptotagmin-1
• Protein Family: Synaptotagmin
• UniProt Gene Name: SYT1
• UniProt Synonym Gene Names: SVP65; SYT
• UniProt Entry Name: SYT1_HUMAN

NCBI Description

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM]

Uniprot Description

SYT1: an integral membrane protein of synaptic vesicles thought to serve as a Ca(2+) sensor in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse. Binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.

Protein type: Vesicle; Calcium-binding; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12cen-q21

Cellular Component: SNARE complex; presynaptic membrane; synaptic vesicle; neuron projection; synaptic vesicle membrane; plasma membrane; integral to membrane; excitatory synapse; cell junction

Molecular Function: protein C-terminus binding; identical protein binding; clathrin binding; calcium-dependent phospholipid binding; transporter activity; calcium ion binding; calcium-dependent protein binding; calmodulin binding; phosphatidylinositol-4,5-bisphosphate binding; SNARE binding; protein binding; low-density lipoprotein receptor binding; phosphatidylserine binding; syntaxin-1 binding; phosphatidylinositol binding

Biological Process: regulation of exocytosis; synaptic transmission; positive regulation of calcium ion-dependent exocytosis; synaptic vesicle exocytosis; vesicle docking; regulation of synaptic transmission, glutamatergic; glutamate secretion; neurotransmitter secretion; positive regulation of vesicle fusion; pathogenesis; detection of calcium ion; positive regulation of synaptic transmission; protein homooligomerization