Rabbit anti-Human, Mouse IER3 Polyclonal Antibody | anti-IER3 antibody

IER3, NT (IER3, DIF2, IEX1, PRG1, Radiation-inducible immediate-early gene IEX-1, Differentiation-dependent gene 2 protein, Immediate early protein GLY96, Immediate early response 3 protein, PACAP-responsive gene 1 protein) (Azide free) (HRP)

Cat. Num. AAA6309215

• Gene Names: IER3; DIF2; IEX1; PRG1; DIF-2; GLY96; IEX-1; IEX-1L
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IER3; Polyclonal Antibody; NT (IER3; DIF2; IEX1; PRG1; Radiation-inducible immediate-early gene IEX-1; Differentiation-dependent gene 2 protein; Immediate early protein GLY96; Immediate early response 3 protein; PACAP-responsive gene 1 protein) (Azide free) (HRP); PACAP-responsive gene 1 protein; anti-IER3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-IER3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: IER3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-73 amino acids from the N-terminal region of human IER3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of IER3 in mouse heart tissue lysate (35ug/lane) using antibodyMBS6006045.)

Western Blot (WB)

(Western blot analysis of IER3 in A549 cell lysate 35ug/lane) using antibodyMBS6006045.)

Immunohistochemistry (IHC)

(IHC staining of FFPE human liver tissue using antibodyMBS6006045. Peroxidase-conjugated secondary antibody and DAB staining were used for visualization. )

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of IER3 in A549 cells. Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) was used as secondary antibody. DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of IER3 in A549 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibody was used.)

Related Product Information for anti-IER3 antibody

This gene functions in the protection of cells from Fas-or tumor necrosis factor type alpha-induced apoptosis. Partially degraded and unspliced transcripts are found after virus infection in vitro, but these transcripts are not found in vivo and do not generate a valid protein.

Product Categories/Family for anti-IER3 antibody

Antibodies; Apoptosis

References

Shahid, M., et al. Hypertension 56(4):705-712(2010). Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010). Fellay, J., et al. PLoS Genet. 5 (12), E1000791 (2009): Do, T.N., et al. Cancer Genet. Cytogenet. 195(1):31-36(2009). Barcellos, L.F., et al. PLoS Genet. 5 (10), E1000696 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 119964723
• NCBI GeneID: 8870
• NCBI Accession #: NP_003888.2
• NCBI GenBank Nucleotide #: NM_003897.3
• UniProt Accession #: P46695; Q5SU30; Q92691; Q93044
• Molecular Weight: 16,903 Da
• NCBI Official Full Name: radiation-inducible immediate-early gene IEX-1
• NCBI Official Synonym Full Names: immediate early response 3
• NCBI Official Symbol: IER3
• NCBI Official Synonym Symbols: DIF2; IEX1; PRG1; DIF-2; GLY96; IEX-1; IEX-1L
• NCBI Protein Information: radiation-inducible immediate-early gene IEX-1; PACAP-responsive gene 1 protein; anti-death protein; differentiation-dependent gene 2 protein; expressed in pancreatic carcinoma; gly96, mouse, homolog of; immediate early protein GLY96; immediate early resp
• UniProt Protein Name: Radiation-inducible immediate-early gene IEX-1
• Protein Family: Radiation-inducible immediate-early gene
• UniProt Gene Name: IER3
• UniProt Synonym Gene Names: DIF2; IEX1; PRG1; Protein DIF-2; Protein PRG1
• UniProt Entry Name: IEX1_HUMAN

NCBI Description

This gene functions in the protection of cells from Fas- or tumor necrosis factor type alpha-induced apoptosis. Partially degraded and unspliced transcripts are found after virus infection in vitro, but these transcripts are not found in vivo and do not generate a valid protein. [provided by RefSeq, Jul 2008]

Uniprot Description

IEX-1: May play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A- PPP2R5C holoenzyme. Acts also as an ERK downstream effector mediating survival. Belongs to the IER3 family.

Protein type: Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: integral to membrane; nucleus

Molecular Function: protein binding

Biological Process: regulation of DNA repair; anatomical structure morphogenesis; response to protozoan; DNA damage response, signal transduction resulting in induction of apoptosis; apoptosis; mitotic cell cycle G2/M transition DNA damage checkpoint; negative regulation of systemic arterial blood pressure; regulation of nucleocytoplasmic transport; negative regulation of inflammatory response; positive regulation of protein catabolic process; negative regulation of glycolysis; negative regulation of apoptosis