Rabbit anti-Human SYT1 Polyclonal Antibody | anti-SYT1 antibody

SYT1, CT (SYT1, SVP65, SYT, Synaptotagmin-1, Synaptotagmin I, p65) (FITC)

Cat. Num. AAA6352965

• Gene Names: SYT1; P65; SYT; SVP65
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SYT1; Polyclonal Antibody; CT (SYT1; SVP65; SYT; Synaptotagmin-1; Synaptotagmin I; p65) (FITC); p65; anti-SYT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SYT1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SYT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 348-377 amino acids from the C-terminal region of human SYT1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SYT1 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the SYT1 antibody detected the SYT1 protein (arrow).)

Immunohistochemistry (IHC)

(SYT1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SYT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-SYT1 antibody

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).

Product Categories/Family for anti-SYT1 antibody

Antibodies; Syntaxin; Synaptotagmin; Synaptophysin

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Musch, M.W., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 298 (2), G203-G211 (2010): Kathir, K.M., et al. Biochim. Biophys. Acta 1798(2):297-302(2010). Pattaro, C., et al. BMC Med. Genet. 11, 41 (2010): Hamdan, F.F., et al. Ann. Neurol. 65(6):748-753(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 209447073
• NCBI GeneID: 6857
• NCBI Accession #: NP_001129278.1; NP_005630.1; NP_001129277.1
• NCBI GenBank Nucleotide #: NM_001135806.1
• UniProt Accession #: P21579; Q6AI31
• Molecular Weight: 47,573 Da
• NCBI Official Full Name: synaptotagmin-1
• NCBI Official Synonym Full Names: synaptotagmin I
• NCBI Official Symbol: SYT1
• NCBI Official Synonym Symbols: P65; SYT; SVP65
• NCBI Protein Information: synaptotagmin-1; sytI; synaptotagmin 1
• UniProt Protein Name: Synaptotagmin-1
• Protein Family: Synaptotagmin
• UniProt Gene Name: SYT1
• UniProt Synonym Gene Names: SVP65; SYT; SytI
• UniProt Entry Name: SYT1_HUMAN

NCBI Description

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010]