Western Blot (WB)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human 293T Cells.)
Rabbit anti-Human Symplekin Polyclonal Antibody | anti-SYMPK antibody
Symplekin (SYMPK) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human 293T Cells.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human 293T Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant SYMPK, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant SYMPK, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Colon Cancer Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Colon Cancer Tissue.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types. [provided by RefSeq, Jul 2008]
Uniprot Description
Symplekin: Heat-labile component of a multimolecular complex that function in histone mRNA 3'-end processing. Specific component of the tight junction (TJ) plaque, but might not be an exclusively junctional component. May have a house-keeping rule. May be required for pre-mRNA polyadenylation. Found in a heat-sensitive complex at least composed of several cleavage and polyadenylation specific and cleavage stimulation factors. Interacts with CPSF2, CPSF3 and CSTF2. Interacts with HSF1 in heat-stressed cells. In testis, expressed in polar epithelia and Sertoli cells but not in vascular endothelia. The protein is detected in stomach, duodenum, pancreas, liver, fetal brain, carcinomas, lens-forming cells, fibroblasts, lymphocytes, lymphoma cells, erythroleukemia cells but not in endothelium of vessels, epidermis, intercalated disks, Purkinje fiber cells of the heart and lymph node. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; RNA processing
Chromosomal Location of Human Ortholog: 19q13.32
Cellular Component: bicellular tight junction; cytoplasm; cytoskeleton; cytosol; nucleoplasm; plasma membrane
Molecular Function: protein binding
Biological Process: cell adhesion; mRNA 3'-end processing; mRNA export from nucleus; mRNA polyadenylation; mRNA splicing, via spliceosome; positive regulation of protein dephosphorylation; termination of RNA polymerase II transcription
Research Articles on SYMPK
Similar Products
Product Notes
The SYMPK sympk (Catalog #AAA2032552) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Symplekin (SYMPK) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Symplekin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the SYMPK sympk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Symplekin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
