Western Blot (WB)
(Fig: 1 Western blot analysis of Survivin in normal prostate epithelium (NPE) and prostate cancer using at 1: 2000. 100 ug/lane of each human tissue lysate was loaded per lane. Survivin was detected in 7 out of 8 prostate cancer patient specimens, but was not detected in NPE.)
Rabbit Survivin Polyclonal Antibody | anti-birc5.1.L antibody
Polyclonal Antibody to Survivin
Gene Names
birc5.1.L; Su2; BIR1; api4; svv1; XBIR1; Xsvv1; epr-1; xsurv; bir1-A; birc5.1; birc5.1-a; birc5.1-b; xSurvivin; XSurvivin1
Reactivity
Rat, Mouse, Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Serum
Synonyms
Survivin; Polyclonal Antibody; Polyclonal Antibody to Survivin; birc5.1-a; bir1-A; svv1; Bir1-A protein; XSurvivin1; Survivin1-a; Antibody to Survivin; Anti-Survivin Antibody; Survivin Antibody; anti-birc5.1.L antibody
Host
Rabbit
Reactivity
Rat, Mouse, Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Serum
Form/Format
50 ul sera
Sequence Length
160
Applicable Applications for anti-birc5.1.L antibody
Immunoprecipitation (IP), Immunohistochemistry (IHC) Formalin/Paraffin, Western Blot (WB)
Application Notes
WB: 1: 1000-1: 2000
IHC (paraffin): 1: 1000-1: 5000
IP: 1: 50-1: 200
IHC (paraffin): 1: 1000-1: 5000
IP: 1: 50-1: 200
Immunogen Information
A full-length recombinant protein of human Survivin was used as immunogen for this antibody
Antigen
Survivin
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
Western Blot (WB)
(Fig: 1 Western blot analysis of Survivin in normal prostate epithelium (NPE) and prostate cancer using at 1: 2000. 100 ug/lane of each human tissue lysate was loaded per lane. Survivin was detected in 7 out of 8 prostate cancer patient specimens, but was not detected in NPE.)
Western Blot (WB)
(Fig: 1 Western blot analysis of Survivin in normal prostate epithelium (NPE) and prostate cancer using at 1: 2000. 100 ug/lane of each human tissue lysate was loaded per lane. Survivin was detected in 7 out of 8 prostate cancer patient specimens, but was not detected in NPE.)
Immunohistochemistry (IHC)
(Fig: 2 Immunohistochemical analysis of Survivin in formalin-fixed, paraffin-embedded human esophageal cancer using at 1: 2000. A1 is a high magnification of A. Hematoxylin-eosin counterstain.)
Immunohistochemistry (IHC)
(Fig: 2 Immunohistochemical analysis of Survivin in formalin-fixed, paraffin-embedded human esophageal cancer using at 1: 2000. A1 is a high magnification of A. Hematoxylin-eosin counterstain.)
Immunohistochemistry (IHC)
(Fig: 3 Immunohistochemical analysis of Survivin in a formalin-fixed, paraffin-embedded human prostate cancer array at 1: 2000. A1 is a high magnification of A. Differential expression of Survivin is seen in tissue cores from four different prostate cancer patients. Hematoxylin-eosin counterstain.)
Immunohistochemistry (IHC)
(Fig: 3 Immunohistochemical analysis of Survivin in a formalin-fixed, paraffin-embedded human prostate cancer array at 1: 2000. A1 is a high magnification of A. Differential expression of Survivin is seen in tissue cores from four different prostate cancer patients. Hematoxylin-eosin counterstain.)
Related Product Information for anti-birc5.1.L antibody
Survivin is a member of the family of inhibitor of apoptosis proteins (IAP), and is involved in both the control of apoptosis and regulation of cell division. IAPs suppress mitochondria-dependent and -independent apoptosis by binding to and inhibiting caspases through their BIR domains. It is a 142 amino acid protein with an approximate molecular weight of 16.5 kDa. Structurally, survivin is composed of a single BIR domain and an extended COOH-terminal a-helical coiled-coil domain and is highly expressed in embryonic and fetal organs. Survivin is undetectable in most normal differentiated and non-proliferating tissues, but is highly expressed in majority of human tumor types including lung, breast, colon, gastric, leukemias, neuroblastoma, brain tumors, pheochromocytoma, soft tissue sarcomas, melanomas and other skin tumors. Additionally, survivin expression has also been detected in a variety of preneoplastic or benign leisons, suggesting that re-expression of survivin may occur early during malignant transformation or following a disruption in the balance between cell division and apoptosis.
Product Categories/Family for anti-birc5.1.L antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
18,686 Da
NCBI Official Full Name
Baculoviral IAP repeat-containing protein 5.1-A
NCBI Official Synonym Full Names
baculoviral IAP repeat-containing 5, gene 1 L homeolog
NCBI Official Symbol
birc5.1.L
NCBI Official Synonym Symbols
Su2; BIR1; api4; svv1; XBIR1; Xsvv1; epr-1; xsurv; bir1-A; birc5.1; birc5.1-a; birc5.1-b; xSurvivin; XSurvivin1
NCBI Protein Information
baculoviral IAP repeat-containing protein 5.1-A
UniProt Protein Name
Baculoviral IAP repeat-containing protein 5.1-A
UniProt Gene Name
birc5.1-a
UniProt Synonym Gene Names
; xSurvivin
Uniprot Description
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Stimulates the mitotic kinase activity of aurkb/aurora-B in the CPC. Does not appear to exhibit anti-apoptotic activity.
Research Articles on birc5.1.L
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Product Notes
The birc5.1.L birc5.1-a (Catalog #AAA668798) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Survivin reacts with Rat, Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's Survivin can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Immunohistochemistry (IHC) Formalin/Paraffin, Western Blot (WB). WB: 1: 1000-1: 2000 IHC (paraffin): 1: 1000-1: 5000 IP: 1: 50-1: 200. Researchers should empirically determine the suitability of the birc5.1.L birc5.1-a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Survivin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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