Rabbit anti-Mouse SURF4 Polyclonal Antibody | anti-SURF4 antibody

SURF4, CT (Surfeit Locus Protein 4, SURF-4, ERV29, FLJ22993, MGC102753) (PE)

Cat. Num. AAA6352675

• Gene Names: SURF4; ERV29
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SURF4; Polyclonal Antibody; CT (Surfeit Locus Protein 4; SURF-4; ERV29; FLJ22993; MGC102753) (PE); Surfeit locus protein 4; anti-SURF4 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SURF4 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SURF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 210-237 amino acids selected from the C-terminal region of human SURF4.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SURF4 Antibody (C-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the SURF4 antibody detected the SURF4 protein (arrow).)

Related Product Information for anti-SURF4 antibody

This gene is located in the surfeit gene cluster, which is comprised of very tightly linked housekeeping genes that do not share sequence similarity. The encoded protein is a conserved integral membrane protein containing multiple putative transmembrane regions. In eukaryotic cells, protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COPs). The specific function of this protein has not been determined but its yeast homolog is directly required for packaging glycosylated pro-alpha-factor into COPII vesicles. This gene uses multiple polyadenylation sites, resulting in transcript length variation. The existence of alternatively spliced transcript variants has been suggested, but their validity has not been determined.

Product Categories/Family for anti-SURF4 antibody

Antibodies; Membrane Proteins

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Mitrovic, S., et al. Mol. Biol. Cell 19(5):1976-1990(2008). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 19557691
• NCBI GeneID: 6836
• NCBI Accession #: NP_149351.1
• NCBI GenBank Nucleotide #: NM_033161.3
• UniProt Accession #: O15260; O60923; Q5T8U6; Q9UNZ0; Q9UNZ1; B7Z6A4
• Molecular Weight: 17,970 Da
• NCBI Official Full Name: surfeit locus protein 4 isoform 1
• NCBI Official Synonym Full Names: surfeit 4
• NCBI Official Symbol: SURF4
• NCBI Official Synonym Symbols: ERV29
• NCBI Protein Information: surfeit locus protein 4
• UniProt Protein Name: Surfeit locus protein 4
• Protein Family: Surfeit locus protein
• UniProt Gene Name: SURF4
• UniProt Synonym Gene Names: SURF-4

NCBI Description

This gene is located in the surfeit gene cluster, which is comprised of very tightly linked housekeeping genes that do not share sequence similarity. The encoded protein is a conserved integral membrane protein that interacts with endoplasmic reticulum-Golgi intermediate compartment proteins. Disruption of this gene results in reduced numbers of endoplasmic reticulum-Golgi intermediate compartment clusters and redistribution of coat protein I to the cytosol. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

May play a role in the maintenance of the architecture of the endoplasmic reticulum-Golgi intermediate compartment and of the Golgi.