Rabbit NLRP3 Polyclonal Antibody | anti-NLRP3 antibody

NLRP3 Antibody

Cat. Num. AAA7128801

• Gene Names: NLRP3; AII; AVP; FCU; MWS; FCAS; CIAS1; FCAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry
• Purity: Antigen Affinity Purification
• Synonyms: NLRP3; Polyclonal Antibody; NLRP3 Antibody; NLR family; pyrin domain containing 3; anti-NLRP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purification
• Form/Format: Liquid; In Phosphate Buffered Saline, pH 7.4, 0.05% NaN3, and 40% Glycerol

• Applicable Applications for anti-NLRP3 antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:1000-1:2000; IHC: 1:25-1:100
• Immunogen: Synthetic peptide of human NLRP3
• Immunogen Species: Human
• Conjugate: Non-conjugated
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)-Paraffin

(The image is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using MBS7128801(NLRP3 Antibody) at dilution 1/25. (Original magnification: ×200))

Immunohistochemistry (IHC)-Paraffin

(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using MBS7128801(NLRP3 Antibody) at dilution 1/25. (Original magnification: ×200))

Product Categories/Family for anti-NLRP3 antibody

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 34878690
• NCBI GeneID: 114548
• NCBI Accession #: NP_899632
• NCBI GenBank Nucleotide #: NM_183395.2
• UniProt Accession #: Q96P20; O75434; Q17RS2; Q59H68; Q5JQS8; Q5JQS9; Q6TG35; Q8TCW0; B2RC97; B7ZKS9; B7ZKT2; B7ZKT3
• Molecular Weight: 115,968 Da
• NCBI Official Full Name: NACHT, LRR and PYD domains-containing protein 3 isoform b
• NCBI Official Synonym Full Names: NLR family, pyrin domain containing 3
• NCBI Official Symbol: NLRP3
• NCBI Official Synonym Symbols: AII; AVP; FCU; MWS; FCAS; CIAS1; FCAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL
• NCBI Protein Information: NACHT, LRR and PYD domains-containing protein 3; cryopyrin; caterpiller protein 1.1; PYRIN-containing APAF1-like protein 1; NACHT, LRR and PYD containing protein 3; cold autoinflammatory syndrome 1 protein; NACHT domain-, leucine-rich repeat-, and PYD-con
• UniProt Protein Name: NACHT, LRR and PYD domains-containing protein 3
• Protein Family: NACHT, LRR and PYD domains-containing protein
• UniProt Gene Name: NLRP3
• UniProt Synonym Gene Names: C1orf7; CIAS1; NALP3; PYPAF1; CLR1.1
• UniProt Entry Name: NALP3_HUMAN

NCBI Description

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq, Oct 2008]

Uniprot Description

NLRP3: May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1); also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS); also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA); also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation. Belongs to the NLRP family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 1q44

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; peptidoglycan binding; ATP binding

Biological Process: caspase activation; apoptosis; negative regulation of acute inflammatory response; positive regulation of caspase activity; interleukin-1 beta production; defense response; negative regulation of NF-kappaB import into nucleus; positive regulation of interleukin-1 beta secretion; signal transduction; protein oligomerization; activation of NF-kappaB transcription factor; negative regulation of interleukin-1 beta secretion; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; interleukin-18 production; innate immune response; interleukin-1 secretion; inflammatory response; detection of biotic stimulus; defense response to virus

Disease: Cinca Syndrome; Familial Cold Autoinflammatory Syndrome 1; Muckle-wells Syndrome