Immunohistochemistry (IHC)
(Immunochemical staining of human SUPT16H in human skin with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human SUPT16H Polyclonal Antibody | anti-SUPT16H antibody
Anti-SUPT16H Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
SUPT16H; Polyclonal Antibody; Anti-SUPT16H Antibody; Rabbit Polyclonal; SUPT16H Antibody; Rabbit PAb; Antigen Affinity Purified; anti-SUPT16H antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human SUPT16H
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-SUPT16H antibody
Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
IHC-P: 1:500-1:5000
ICC/IF: 1:50-1:200
ICC/IF: 1:50-1:200
Immunogen
E Coli-derived Human SUPT16H fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human SUPT16H fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human SUPT16H in human skin with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human SUPT16H in human skin with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
Immunofluorescence (IF)
(Immunofluorescence staining of SUPT16H in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human SUPT16H polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of SUPT16H in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human SUPT16H polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Related Product Information for anti-SUPT16H antibody
The histone chaperone FACT (facilitates chromatin transcription) is a large heterodimeric complex with roles in transcription, replication, and repair. FACT promotes and subsequently restricts access to DNA as a result of dynamic nucleosome reorganization. The human FACT complex is composed of two polypeptides identified as Spt16 (Suppressor of ty 16) and SSRP1 (structure-specific recognition protein 1) that are both essential for nucleosome reorganization. The Spt16 protein is divided into three distinct structural domains and a highly acidic intrinsically disordered C-terminal domain required for alleviation of nucleosome blockage during polymerase II elongation. The SSRP1 component of FACT is divided into five well-defined domains, which includes a C-terminal HMG-1 DNA binding domain.
References
Winkler DD, et al. (2011) Histone chaperone fact coordinates nucleosome interaction through multiple synergistic binding events. J Biol Chem 286 (48): 41883-41892.
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Product Notes
The SUPT16H (Catalog #AAA8110074) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SUPT16H Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SUPT16H can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF). IHC-P: 1:500-1:5000 ICC/IF: 1:50-1:200. Researchers should empirically determine the suitability of the SUPT16H for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SUPT16H, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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