Immunohistochemistry (IHC)
(Immunochemical staining of human CHD5 in cynomolgus cerebellum with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human CHD5 Polyclonal Antibody | anti-CHD5 antibody
Anti-CHD5 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
CHD5; Polyclonal Antibody; Anti-CHD5 Antibody; Rabbit Polyclonal; CHD5 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-CHD-5 Antibody; Anti-RP1-233K16.2 Antibody; anti-CHD5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CHD5
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-CHD5 antibody
Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
IHC-P: 1:200-1:1000
ICC/IF: 1:100-1:500
ICC/IF: 1:100-1:500
Immunogen
E Coli-derived Human CHD5 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human CHD5 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human CHD5 in cynomolgus cerebellum with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human CHD5 in cynomolgus cerebellum with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunofluorescence (IF)
(Immunofluorescence staining of CHD5 in PC3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CHD5 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of CHD5 in PC3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CHD5 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.)
Related Product Information for anti-CHD5 antibody
Chromodomain helicase DNA binding protein 5 (CHD5) is a member of CHD protein family which acts in chromatin organization, regulation of transcription and also genomic stability and cancer prevention. CHD5 functions by forming a nucleosome remodeling and deacetylation (NuRD) complex that regulates the transcription of particular genes. CHD5 is preferentially expressed in the nervous system and testis. CHD5 acts as a tumor suppressor n gliomas and a variety of other tumor types, including breast, colon, lung, ovary, and prostate cancers. CHD5 may serve as an independent prognostic biomarker for colorectal patients.
References
Zhou Y, et al. (2018) Expression of chd5 may serve as an independent biomarker of prognosis in colorectal cancer via immunohistochemical staining. Int J Clin Exp Pathol 11 (5): 2792-2798.Xiao Q, et al. (2018) A rare chd5 haplotype and its interactions with environmental factors predicting hepatocellular carcinoma risk. BMC Cancer 18 (1): 658.Kolla V, et al. (2014) Role of chd5 in human cancers: 10 years later. Cancer Res 74 (3): 652-658.
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Product Notes
The CHD5 (Catalog #AAA8110091) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CHD5 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHD5 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF). IHC-P: 1:200-1:1000 ICC/IF: 1:100-1:500. Researchers should empirically determine the suitability of the CHD5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHD5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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